
Amount : | Non Profit |
Langerin Stable Cell Line is a stably transfected CHO-K1 cell line which expresses human Langerin, also known as CD207.
Sequence data: hLangerin (accession numberNP_056532)
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
Application:.
- Screen for antibodies of human Langerin through Flow Cytometry.
Culture conditions:
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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最近开始做TUNEL染色,第一次按罗氏protocol做的,阴性对照是没有显色的。没做阳性对照。第二次、第三次做出来背景都很深,肾小管每个细胞核上都染上了棕黄色,DAB染色很快,几秒就变一片黄。具体步骤是:1烤片脱蜡至水2.蛋白酶K20ug/ML37℃10分钟3.tunel液冰上配置后加组织上,37度1h。4.pod液37℃30min5.DAB显色。在蚂蚁淘中检索后,优化了实验条件,在蛋白酶K前加了3%H2O2封闭10min,缩短了tunel液至40min。这是第三次的结果大家帮我看看
这是对照组
这是模型组
Bone Mesenchymal Stem Cells 作为一个细胞群体,还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法,就是采用培养,colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数,比如STRO-1,但是一般认为STRO-1阳性的细胞更趋向于造血干细胞,和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf

