The Premix WST-1 Cell Proliferation Assay System allows fast and easy colorimetric measurement of cell proliferation and viability in a 96-well format, without radioactive isotopes or organic solvents. The Premix WST-1 Cell Proliferation Assay System is also applicable to cytotoxicity and inhibitory assays, where dye production decreases rather than increases.
The Premix WST-1 Cell Proliferation Assay System allows fast and easy colorimetric measurement of cell proliferation and viability in a 96-well format, without radioactive isotopes or organic solvents. The Premix WST-1 Cell Proliferation Assay System is also applicable to cytotoxicity and inhibitory assays, where dye production decreases rather than increases.
The colorimetric assay is based on the cleavage of the tetrazolium salt WST-1 to a formazan-class dye by mitochondrial succinate-tetrazolium reductase in viable cells. As the cells proliferate, more WST-1 is converted to the formazan product. The quantity of formazan dye is directly related to the number of metabolically active cells, and can be quantified by measuring absorbance at 420–480 nm (Amax 450 nm) in a multiwell plate reader.
An effortless protocol
No washing, harvesting, or solubilization steps are required with the Premix WST-1 Cell Proliferation Assay System. The entire procedure, from cell culture to data analysis, can be carried out with a multiwell plate reader in the same 96-well plate.
Once your cells are cultured, the assay can be completed in approximately 1 hour. Simply add the Premixed WST-1 Cell Proliferation Reagent to the plate, culture your cells for at least 30 min in a humidified atmosphere (up to 4 hr, depending on cell type and density) and measure the absorbance. The colored product is generated continuously, so you can monitor the progress of your experiment over time by repeatedly reading the plate and returning it to the incubator for further development. This eliminates guesswork and ensures that the optimal assay endpoint is not overlooked.
Stable reagents
Unlike traditional WST-1 assay reagents, which can only be stored for three days at 4°C or one month at –20°C, the Premixed WST-1 Cell Proliferation Reagent has been formulated for increased stability: it can be stored for two weeks at 4°C or one year at –20°C. It is supplied as a ready-to-use solution containing WST-1 and an electron-coupling reagent, diluted in sterile phosphate-buffered saline.
Non-radioactive, colorimetric assay
Many cell proliferation assays are based on measurements of radioactively labeled nucleoside uptake during DNA synthesis. The Premixed WST-1 Cell Proliferation Reagent assay is nonradioactive, which enhances convenience and laboratory safety. Furthermore, the Premixed WST-1 Cell Proliferation Reagent is highly water-soluble compared to other tetrazolium salts used in similar assays, which may require surfactants or organic solvents for dissolution.
The Premixed WST-1 Cell Proliferation Reagent will simplify and streamline your cell proliferation assays. The simple, one-solution protocol saves time and is easy to use in a 96-well plate format. Plates can be read and returned to the incubator for further development to simplify time-course experiments.
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Bone Mesenchymal Stem Cells 作为一个细胞群体，还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法，就是采用培养，colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数，比如STRO-1，但是一般认为STRO-1阳性的细胞更趋向于造血干细胞，和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
有没有朋友使用碧云天的细胞衰老β-半乳糖苷酶染色试剂盒做过细胞衰老，本人小白，求相关细胞处理的过程及数据分析方法。贴壁细胞六孔板需要接种多少，染色后怎样计数进行数据处理。谢谢啦！
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注，图注的意思我明白，但是我想知道merge后的图看什么颜色的荧光，蓝色是细胞核，红色是lysosome(位于胞质），绿色是AMPKβ2，该实验是想观察AMPKβ2是否转位到lysosome上了，如果确实发生了AMPKβ2转位到lysosome上，那么merge后是红色与绿色融合在一起，是吗？融合在一起发什么颜色的光了？
