Liposome formulations can be broadly divided into cationic, anionic and neutral subtypes. Charged liposomes are used in many different types of studies such as, blood complement studies, gene transfer studies and other biomedical applications to name a few. Cellsome® liposomes made from anionic PEGylated lipids provide certain benefits.
Anionic liposomes offer a number of attributes, relative to the challenges associated with cationic formulations such as, the administration of cationic liposomes results in higher cytotoxicity levels, both in vitro (Zhong et al., 2009) and in vivo (Balazs and Godbey, 2010). Anionic liposomes demonstrate greater stability in solution (Zhigaltsev et al., 2002, Phillips and Heydari, 1996), lead to lower aggregation when compared with neutrally charged liposomes, and have increased endocytosis when compared with cationic (Bajoria et al., 1997) and neutral liposomes.
To improve liposome stability and enhance their circulation times in the blood, a sterically-stabilized, hydrophilic polymer, polyethylene glycol (PEG), has been shown to be the optimal choice for obtaining sterically-stabilized liposomes. Using an Anionic Liposome with PEG will protect the liposomes from circulating proteins, improving their plasma clearance and enhancing their therapeutic effects.
The four products provided that are made from Anionic PEGylated Lipids provided here consist of either DSPC:Chol:DSPG:DSPE-PEG(2000) (60:30:5:5 molar ratio), DOPC:Chol:DOPG:DOPE-PEG(2000) (60:30:5:5 molar ratio), DSPC:Chol:DSPS:DSPE-PEG(2000) (60:30:5:5 molar ratio), or DOPC:Chol:DOPS:DOPE-PEG(2000) (60:30:5:5 molar ratio).
ebiomall.com
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Bone Mesenchymal Stem Cells 作为一个细胞群体，还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法，就是采用培养，colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数，比如STRO-1，但是一般认为STRO-1阳性的细胞更趋向于造血干细胞，和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注，图注的意思我明白，但是我想知道merge后的图看什么颜色的荧光，蓝色是细胞核，红色是lysosome(位于胞质），绿色是AMPKβ2，该实验是想观察AMPKβ2是否转位到lysosome上了，如果确实发生了AMPKβ2转位到lysosome上，那么merge后是红色与绿色融合在一起，是吗？融合在一起发什么颜色的光了？
