
Overview:
IRS1 is the substrate for the insulin tyrosine kinase receptor and is found in a variety of insulin-responsive cells and tissues. IRS1 protein has no intrinsic enzymatic activity but acts as a docking protein, via the SH2 domains, for mediating the insulin downstream signaling events. IRS1 has been shown to associate with the 14-3-3 family of proteins and this could play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (1). IRS1 may be associated with colorectal cancer and diet and related factors may affect the risk by modifying plasma insulin levels. Thus, the inter-individual variation in insulin signaling mediated by IRS1 may play a plausible role in the development of colorectal cancer (2).
References:
1. Ogihara, T. et al: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J. Biol. Chem. 272: 25267-25274, 1997. 2. Slattery, M.L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in outhwestern United States. Breast Cancer Res Treat. 2007.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
二、补体(complement,C)是存在于正常人和动物血清与组织液中的一组经活化后具有酶活性的蛋白质。早在19世纪末Bordet即证实,新鲜血液中含有一种不耐热的成分,可辅助和补充特异性抗体,介导免疫溶菌、溶血作用,故称为补体。补体是由30余种可溶性蛋白、膜结合性蛋白和补体受体组成的多分子系统,故称为补体系统(complementsystem)。根据补体系统各成分的生物学功能,可将其分为补体固有成分、补体调控成分和补体受体(CR)。
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白,因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出,如果你的一抗是小鼠IgM,那么相应的二抗就应当是抗小鼠IgM,或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类(IgG1,IgG2a,IgG2b,IgG3),那么几乎所有的抗小鼠IgG都可以与之结合,或者你也可以选择专门针对这一亚类的二抗,例如,如果你的一抗是小鼠IgG1,那么你可以选择抗IgG1的二抗,此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类,那么抗小鼠IgG是一个不错的选择,因为此种抗体可以识别大多数类型的IgG免疫球蛋白。

