| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human GFI1 |
| Purification | Affinity purified |
| Peptide Sequence | Synthetic peptide located within the following region: KKAHSYHQPRSPGPDYSLRLENVPAPSRADSTSNAGGAKAEPRDRLSPES |
| Concentration | Batch dependent within range: 100 ul at 0.5 - 1 mg/ml |
| Blocking Peptide | For anti-GFI1 (ARP97709_P050) antibody is Catalog # AAP97709 |
| Datasheets/Manuals | Printable datasheet for anti-GFI1 (ARP97709_P050) antibody |
| Gene Symbol | GFI1 |
|---|---|
| Official Gene Full Name | growth factor independent 1 transcriptional repressor |
| Alias Symbols | SCN2, GFI-1, GFI1A, ZNF163 |
| NCBI Gene Id | 2672 |
| Protein Name | SCN2, GFI-1, GFI1A, ZNF163 |
| Description of Target | This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. |
| Swissprot Id | Q99684 |
| Protein Accession # | NP_001120687.1 |
| Nucleotide Accession # | NM_001127215.1 |
| Protein Size (# AA) | 422 |
| Molecular Weight | 46 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express GFI1.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express GFI1.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
How long will it take to receive "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
What buffer format is "GFI1 Antibody - N-terminal region (ARP97709_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
This target may also be called "SCN2, GFI-1, GFI1A, ZNF163" in publications.
What is the shipping cost for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "46 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "GFI1 Antibody - N-terminal region (ARP97709_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "GFI1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "GFI1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "GFI1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "GFI1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "GFI1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "GFI1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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二、补体(complement，C)是存在于正常人和动物血清与组织液中的一组经活化后具有酶活性的蛋白质。早在19世纪末Bordet即证实，新鲜血液中含有一种不耐热的成分，可辅助和补充特异性抗体，介导免疫溶菌、溶血作用，故称为补体。补体是由30余种可溶性蛋白、膜结合性蛋白和补体受体组成的多分子系统，故称为补体系统（complementsystem）。根据补体系统各成分的生物学功能，可将其分为补体固有成分、补体调控成分和补体受体（CR）。
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白，因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出，如果你的一抗是小鼠IgM，那么相应的二抗就应当是抗小鼠IgM，或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类（IgG1，IgG2a，IgG2b，IgG3），那么几乎所有的抗小鼠IgG都可以与之结合，或者你也可以选择专门针对这一亚类的二抗，例如，如果你的一抗是小鼠IgG1，那么你可以选择抗IgG1的二抗，此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类，那么抗小鼠IgG是一个不错的选择，因为此种抗体可以识别大多数类型的IgG免疫球蛋白。
