| 3,3',5'-Triiodo-L-thyronineTRΔα1 activator |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
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- Purity = 98.49%
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Chemical structure
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| Cas No. | 5817-39-0 | SDF | Download SDF |
| Synonyms | Reverse T3,rT3,3,3’,5’-T3 | ||
| Chemical Name | O-(4-hydroxy-3,5-diiodophenyl)-3-iodo-L-tyrosine | ||
| Canonical SMILES | IC1=C(O)C(I)=CC(OC2=CC=C(C[C@H](N)C(O)=O)C=C2I)=C1 | ||
| Formula | C15H12I3NO4 | M.Wt | 651.0 |
| Solubility | ≥32.55mg/mL in DMSO | Storage | Store at -20°C |
| Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
3,3",5"-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3"-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3",5"-Triiodo-L-thyronine is thyroid hormone receptors TRα and TRβ antagonist [1].
Thyroid hormones play important roles in the development of the mammalian brain by acting on migration and differentiation of neural cells, synaptogenesis, and myelination. The actions of thyroid hormones are mediated by nuclear thyroid hormone receptors (TRs) and regulation of gene expression. TRs have been also involved in adult brain function. In adult mice, TRα1 deletion and a dominant negative mutant receptor expression induce consistent behavioral changes leading to severe anxiety and morphological changes in the hippocampus [2].
The rT3 was about 1,000-fold less active at the thyroid hormone receptors TRα and TRβ compared with 3,3",5-triiodo-L-thyronine and commonly described as inactive [1].It has been reported that rT3 activates a native form of TRα, TRΔα1, which lacks a nuclear localization signal and functions in the cytoplasm. Through this action, rT3 initiates actin polymerization, particularly in astrocytes and neurons [3].
References:[1] Nicod P, Burger A, Staeheli V, et al. A radioimmunoassay for 3, 3′, 5′-triiodo-L-thyronine in unextracted serum: method and clinical results[J]. The Journal of Clinical Endocrinology & Metabolism, 1976, 42(5): 823-829.[2] Bernal J. Thyroid hormone receptors in brain development and function[J]. Nature clinical practice Endocrinology & metabolism, 2007, 3(3): 249-259.[3] Senese R, Cioffi F, De Lange P, et al. Thyroid: biological actions of ‘nonclassical’thyroid hormones[J]. Journal of Endocrinology, 2014, 221(2): R1-R12.
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确定了需要检测样品的种属，就可以选择对应的一抗
而二抗是根据一抗来选择的，比如一抗是小鼠来源的，二抗就要是抗小鼠的
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白，因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出，如果你的一抗是小鼠IgM，那么相应的二抗就应当是抗小鼠IgM，或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类（IgG1，IgG2a，IgG2b，IgG3），那么几乎所有的抗小鼠IgG都可以与之结合，或者你也可以选择专门针对这一亚类的二抗，例如，如果你的一抗是小鼠IgG1，那么你可以选择抗IgG1的二抗，此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类，那么抗小鼠IgG是一个不错的选择，因为此种抗体可以识别大多数类型的IgG免疫球蛋白。
