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BIOMYC-3 is based on the antibiotic ciprofloxacin, which is a member of the fluoroquinolone group and inhibits synthesis of the enzyme DNA gyrase which is responsible for supercoiling in bacterial DNA. Many mycoplasma species have been found to be sensitive to BIOMYC-3, including A. laidlawii, M. orale, M. hyorhinis, M. fermentans, and M. arginini. These species are responsible for most mycoplasma contamination in cell culture. At the concentrations recommended for use, no cytotoxic effects have been found so far, and the treatment is quite easy to perform.The treatment consists of adding the antibiotic to the growth medium for 12 days, and changing the medium every three days. On completion of the treatment, the cells should be grown on media without antibiotics for at least two weeks and then re-tested for the presence of mycoplasma. If the culture tests positive, the course of treatment should be repeated. Treatment with ciprofloxacin does not damage or alter the cells. This antibiotic should not be used repeatedly in cell cultures; otherwise, the mycoplasmas are liable to develop resistance to it.
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不过也有用 HRP标记的protein a或g的,前提是你跑的样里没有IgG之类的东西。
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白,因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出,如果你的一抗是小鼠IgM,那么相应的二抗就应当是抗小鼠IgM,或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类(IgG1,IgG2a,IgG2b,IgG3),那么几乎所有的抗小鼠IgG都可以与之结合,或者你也可以选择专门针对这一亚类的二抗,例如,如果你的一抗是小鼠IgG1,那么你可以选择抗IgG1的二抗,此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类,那么抗小鼠IgG是一个不错的选择,因为此种抗体可以识别大多数类型的IgG免疫球蛋白。

