
- SynonymMSP1D1
- SourceHuman MSP1D1, His Tag (APO-H51H3) is expressed from E.coli cells. It contains AA Gly 1- Gln 211.Predicted N-terminus: HisRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 24.7 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE). This protein is used together with CD20 nanodisc protein (CD0-H52H1) as isotype control.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human MSP1D1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

2e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 10 μg/mL of Human MSP1D1, His Tag (Cat. No. APO-H51H3) and negative control antibody respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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谢谢各位
非常急!
①在用超滤除白蛋白,IgG中硫酸铵盐时,膜包该如何选择?选择几个?
②如用凝胶过滤来分级纯化血清中65%硫酸铵盐析出的白蛋白和纯化33%硫酸铵盐析出的IgG,其填充介质应选择什么?其洗脱缓冲液应用什么好?
下面有些关于这方面的数据,供参考:
①牛血清白蛋白:分子量:66210;分子形状:椭圆形;分子大小:
40Å*140Å;等电点:4.7;血浆中的含量:52.0g/L。
②IgG:分子量:15300;分子形状:球状;等电点:5.8—7.3;血浆中含量:2.0g/L。
③另外,我从书上看到说:凝胶过滤在分级方法中分辨率为中等,但对脱盐效果优良;流速较低,对分级每周期约≥8小时,对脱盐仅30分钟;适用于大规模纯化的最后步骤,在纯化过程的任何阶段均可进行脱盐处理,尤其适用于两种缓冲液交替时。
期待您的帮助,谢谢您。
(一)高密度脂蛋白胆固醇(HDL-C)
【参考值】
0.94~2.0mmol/L
【临床意义】
降低具有临床意义。HDL-C与TG呈负相关系,见于冠心病、动脉粥样硬化、糖尿病、肝脏损害、肾病综合征。
(二)低密度脂蛋白胆固醇(LDL-C)
【参考值】
沉淀法:2.07~3.12mmol/L,3.15~3.61mmol/L为边缘升高,≥3.64mmol/L为升高。
【临床意义】
升高具有临床意义。LDL-C升高与冠心病发病呈正相关系。
(三)脂蛋白(a),LP(a)
【参考值】 <300mg/L
【临床意义】
脂蛋白(a)升高已作为冠心病及脑血管疾病发病的独立危险因素。

