
- SynonymSpike,S protein,Spike glycoprotein,S glycoprotein,COVID-19
- SourceSARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (SPN-C52H6) is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectivelyPredicted N-terminus: Val 16
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 137.8 kDa.
- Endotoxin
- Formulation
Please contact us for detailed information.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
- BackgroundIt"s been reported that Coronaviruscan infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
谢谢各位
非常急!
①在用超滤除白蛋白,IgG中硫酸铵盐时,膜包该如何选择?选择几个?
②如用凝胶过滤来分级纯化血清中65%硫酸铵盐析出的白蛋白和纯化33%硫酸铵盐析出的IgG,其填充介质应选择什么?其洗脱缓冲液应用什么好?
下面有些关于这方面的数据,供参考:
①牛血清白蛋白:分子量:66210;分子形状:椭圆形;分子大小:
40Å*140Å;等电点:4.7;血浆中的含量:52.0g/L。
②IgG:分子量:15300;分子形状:球状;等电点:5.8—7.3;血浆中含量:2.0g/L。
③另外,我从书上看到说:凝胶过滤在分级方法中分辨率为中等,但对脱盐效果优良;流速较低,对分级每周期约≥8小时,对脱盐仅30分钟;适用于大规模纯化的最后步骤,在纯化过程的任何阶段均可进行脱盐处理,尤其适用于两种缓冲液交替时。
期待您的帮助,谢谢您。
(一)高密度脂蛋白胆固醇(HDL-C)
【参考值】
0.94~2.0mmol/L
【临床意义】
降低具有临床意义。HDL-C与TG呈负相关系,见于冠心病、动脉粥样硬化、糖尿病、肝脏损害、肾病综合征。
(二)低密度脂蛋白胆固醇(LDL-C)
【参考值】
沉淀法:2.07~3.12mmol/L,3.15~3.61mmol/L为边缘升高,≥3.64mmol/L为升高。
【临床意义】
升高具有临床意义。LDL-C升高与冠心病发病呈正相关系。
(三)脂蛋白(a),LP(a)
【参考值】 <300mg/L
【临床意义】
脂蛋白(a)升高已作为冠心病及脑血管疾病发病的独立危险因素。

