- Description
- Additional
Description
Details
RACTIVITY | Human |
SENSITIVITY | 0.005 mg/L |
ASSAY RANGE | 0.005 mg/L- 10 mg/L |
REAGENTS PROVIDED | CRP MICROTITER PLATECRP CONJUGATECRP STANDARD SETTMB SUBSTRATESTOP SOLUTIONCRP SAMPLE DILUENT |
INTENDED USE
This High Sensitivity Human CRP ELISA kit is to be used for the in vitro quantitative determination of human c reactive protein (CRP) concentrations in serum, plasma and cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE only and not for use in diagnostic or therapeutic procedures.
INTRODUCTION
Human CRP is a kind of nonimmunoglobulin serum substance, a heat labile b-globulin. It is classified in a superfamily of proteins termed pentaxins or pentraxins: cyclic, non-glycosylated structures composed of five apparently identical globular non-covalently linked subunits aggregated symmetrically. Each subunit is 23.05 kD (206 amino acids), with a total molecular weight of 117.5 kDa, and consists of 14 anti-parallel b-strands arranged in two b-sheets.
CRP is an acute phase protein, originally identified and named for its ability to precipitate the C-polysaccharide of pneumococcus in the presence of calcium. It is the prototypic acute phase reactant whose presence in plasma or serum serves as a useful laboratory indicator of systemic inflammatory disease. Normally, CRP in human biological fluids is present in trace amounts (0.07-8.00 mg/L, median 0.6 mg/L). Stimulated by certain cytokines (IL-1a, IL-1b, TNF-a and b, and indirectly by IL-6), its synthesis by hepatocytes enhanced dramatically. During the acute phase response, CRP concentration can increase up to 1000-fold within a few hours.
Among acute phase proteins, CRP is a fast-reacting, sensitive and the most easily measured one. It has a rapid response time, short half-life and large incremental change and its catabolism is not affected by the type of inflammation. Following acute tissue damage or during the course of infectious and non-infectious conditions, hepatic synthesis of CRP dramatically increases. Typically, mild elevations of CRP are seen in a variety of inflammatory conditions. Serum amyloid A (SAA) is another major acute phase protein whose response is highly correlated with that of CRP. Both CRP and SAA respond sensitively to several stimuli, but they differ in certain responses.
CITATIONS
1.Mong MC, Hsia TC, Yin MC. Dietary trans fats enhance doxorubicin-induced cardiotoxicity in mice. J Food Sci. 2013 Oct;78(10):H1621-8.
2.Lin MC, Yin MC et al. Preventive effects of ellagic acid against doxorubicin-induced cardio-toxicity in mice. Cardiovasc Toxicol. 2013 Sep;13(3):185-93.
3.White LJ et al. Cardiovascular/non-insulin-dependent diabetes mellitus risk factors and intramyocellular lipid in healthy subjects: a sex comparison. Metabolism. 2006 Jan;55(1):128-34.
4.Franiak-Pietryga I et al. Anti-inflammatory and hypolipemic effects in vitro of simvastatin comparing to epicatechin in patients with type-2 hypercholesterolemia. Food Chem Toxicol. 2009 Feb;47(2):393-7.
5.Liu WH, Liu TC, Yin MC. Beneficial effects of histidine and carnosine on ethanol-induced chronic liver injury. Food Chem Toxicol. 2008 May;46(5):1503-9.
6.Gabriele Wehrwein et al. Lipopolysaccharide regulated protein expression is only partly impaired in monocytes from patients with type I diabetes. Cardiovasc Diabetol. 2006; 5: 5.
7.Sabine Abke et al. Adiponectin-induced secretion of interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1, CCL2) and interleukin-8 (IL-8, CXCL8) is impaired in monocytes from patients with type I diabetes. Cardiovasc Diabetol. 2006; 5: 17.
8. Lin CY et al. Anticoagulatory, antiinflammatory, and antioxidative effects of protocatechuic acid in diabetic mice. J Agric Food Chem. 2009 Aug 12;57(15):6661-7.
9.Teoh H et al. Impaired endothelial function in C-reactive protein overexpressing mice. Atherosclerosis. 2008 Dec;201(2):318-25.
10.Reilly L, Nausch N, Midzi N, Mduluza T, Mutapi F.Association between Micronutrients (Vitamin A, D, Iron) and Schistosome-Specific Cytokine Responses in Zimbabweans Exposed to Schistosoma haematobium. J Parasitol Res 2012; (2012):128628.
11.Khan A, Ali Z.Normal Ranges for Acute Phase Reactants (Interleukin-6, Tumour Necrosis Factor-alpha and C-reactive Protein) in Umbilical Cord Blood of Healthy Term Neonates at the Mount Hope Women"s Hospital, Trinidad. West Indian Med J 2014; 5(63):465-9.
12. Emokpae MA, Aruomaren A, Osime E.Relationship between Neutrophil-to-Lymphocyte Ratio and Inflammatory Markers in Sickle Cell Anaemia Patients with Proteinuria. Med Sci (Basel) 2016; 3(4):.
13. Chen LC, Hu LH, Yin MC.Alleviative effects from boswellic acid on acetaminophen-induced hepatic injury. Biomedicine (Taipei) 2017; 7(2):13.
Additional
Additional Information
| Product Specificity | High Sensitivity Human CRP ELISA Kit |
|---|---|
| Application | Refer to Insert |
| Size | 96 wells |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
一、分离脂蛋白
血浆是分离纯化脂蛋白的首选标本。从血浆（血清）中可分离得到CM、VLDL、LDL和HDL，再经脱脂除去脂蛋白中的脂质，剩下的部分为载脂蛋白的混合物。这一操作过程是获得载脂蛋白的最佳方法。
二、脱脂
由于各种脂蛋白含脂的种类和质量不同，脱脂剂组成略有差异。目前常用的几种脱脂方法有如下几种。
1．Warnick等法
将纯化的脂蛋白慢慢加入到预冷（-20℃）的丙酮：无水乙醇（1：1，V/V）混合液中，脱脂液的量为样品的20倍，不断搅拌，然后在-20℃冰箱放置过夜，4℃离心沉淀，将沉淀重复脱脂一次，用氮气吹干或真空干燥，脱脂物0℃贮存待用。
2．Scanu等法
将脂蛋白加入到预冷的（-15℃）无水乙醇：无水乙醚（3：2）混合液中，不断搅拌，以12h过夜，在-10℃或4℃离心（2000r/min），去上清留沉淀，再重复脱脂一次。用N2或真空干燥，0℃贮存待用。
蛋白质在水溶液中的溶解度是由蛋白质周围亲水基团与水形成水化膜的程度，以及蛋白质分子带有电荷的情况决定的。当用中性盐加入蛋白质溶液，中性盐对水分子的亲和力大于蛋白质，于是蛋白质分子周围的水化膜层减弱乃至消失。同时，中性盐加入蛋白质溶液后，由于离子强度发生改变，蛋白质表面电荷大量被中和，更加导致蛋白溶解度降低，使蛋白质分子之间聚集而沉淀。一般33%硫酸铵为沉淀Ig-G的最适饱和度
