Addgene/pAAV-CAG-GFP/1unit/37825-AAV2.T_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

商品信息

联系客服

郑重提醒：

无质量问题不接受退换货，下单前请仔细核对信息。

下单后请及时联系客服核对商品价格，订单生效后再付款。

Addgene/pAAV-CAG-GFP/1unit/37825-AAV2.T

品牌 /

Addgene

货号 /

37825-AAV2.T

官网地址

美元价：

登录查看

(友情提示：该价格仅为参考，欢迎联系客服询价！)

数量：

免费咨询热线

4000-520-616

商品介绍售后保障相关文章商品咨询

联系客服

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	37825	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
AAV1	37825-AAV1	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV1 trial size	37825-AAV1.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV2	37825-AAV2	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV2 trial size	37825-AAV2.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV5	37825-AAV5	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV5 trial size	37825-AAV5.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV8	37825-AAV8	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV8 trial size	37825-AAV8.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV9	37825-AAV9	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV9 trial size	37825-AAV9.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV Retrograde	37825-AAVrg	Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$380	Add to Cart
AAV Retrograde trial size	37825-AAVrg.T	Virus (20 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information		$130	Add to Cart
AAV PHP.eB	37825-PHPeB	Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information		$380	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
AAV with CAG promoter
(Search Vector Database)
Backbone manufacturer
Scott Sternson
Backbone sizew/o insert(bp)4722
Vector type
Mammalian Expression, AAV ; Adeno-associated virus

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
30°C
Growth Strain(s)
NEB Stable
Growth instructions
Use recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
Copy number
Low Copy

Gene/Insert

Gene/Insert name
GFP
Alt name
Green fluorescent protein
Species
A. victoria
Insert Size (bp)
720
Mutation
N/A
PromoterCAG

Cloning Information

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteEcoRI(not destroyed)
5′ sequencing primeraaagagacagcaaccagg
3′ sequencing primercggcttctggcgtgtgac

Resource Information

Supplemental Documents
- pAAV-CAG-GFP.gb
Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 12 References

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.

Information for AAV1 (Catalog # 37825-AAV1)(Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV1 trial size (Catalog # 37825-AAV1.T)(Back to top)

Purpose

Ready-to-use AAV1 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV1
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 (Catalog # 37825-AAV2)(Back to top)

Purpose

Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV2 trial size (Catalog # 37825-AAV2.T)(Back to top)

Purpose

Ready-to-use AAV2 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV2
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 (Catalog # 37825-AAV5)(Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV5 trial size (Catalog # 37825-AAV5.T)(Back to top)

Purpose

Ready-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV5
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 (Catalog # 37825-AAV8)(Back to top)

Purpose

Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV8 trial size (Catalog # 37825-AAV8.T)(Back to top)

Purpose

Ready-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV8
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 (Catalog # 37825-AAV9)(Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV9 trial size (Catalog # 37825-AAV9.T)(Back to top)

Purpose

Ready-to-use AAV9 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
BufferPBS + 0.001% Pluronic F-68
SerotypeAAV9
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Information for AAV Retrograde (Catalog # 37825-AAVrg)(Back to top)

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
Titer≥ 7×10¹² vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Data submitted about 37825-AAVrg by requesting scientist(s):

Data 1: Guinea pig, Brain parenchyma

Information for AAV Retrograde trial size (Catalog # 37825-AAVrg.T)(Back to top)

Purpose

Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

Delivery

Volume20 µL
Titer≥ 7×10¹² vg/mL
Pricing$100 USD for preparation of 20 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
SerotypeAAV retrograde (AAVrg)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.

Information for AAV PHP.eB (Catalog # 37825-PHPeB)(Back to top)

Purpose

Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA.

CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Delivery

Volume100 µL
TiterEnter the titer here≥ 1×10¹³ vg/mL
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
StorageStore at -80℃. Thaw just before use and keep on ice.
ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)
BufferPBS + 0.001% Pluronic F-68
SerotypePHPeB (plasmid #103005)
PurificationIodixanol gradient ultracentrifugation
Reporter GeneGFP

Biosafety

Resource Information

Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
Industry Terms
- Not Available to Industry

Viral Quality Control

Quality Control:

Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for moreinformation.

Addgene Comments

Citation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.

蚂蚁淘电商平台
ebiomall.com

公司简介

蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...

蚂蚁淘承诺

正品保证： 全球直采在线追溯蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权；同时客户可以通过订单详情查看到货物从厂方至客户的所有流程，确保货物的来源；正规报关，提供13%增值税发票。

及时交付： 限时必达畅选无忧蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上，部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。

轻松采购： 在线下单简单省事蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；报价单与合同可以直接在线生成或打印；就像在京东购物一样，您的鼠标点击几次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。

售后申请： 耐心讲解优质服务蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时，您可通过我的订单提交您的“申请售后”，蚂蚁淘产品顾问会第一时间为您处理，在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。

石蜡切片茜素红(ALIZARIN RED S)钙染色试剂盒

2021-08-31

Chemokine品牌细胞因子/免疫试剂价格/厂家直采/进口查看更多>

血清总蛋白测定的临床意义

2021-09-03

血清蛋白质是各种蛋白的复杂混合物。可利用不同的方法将其分离。血浆中的白蛋白、a1、a2、β球蛋白，纤维蛋白原，凝血酶原和其它凝血因子等均由肝细胞合成。γ球蛋白主要来自浆细胞。当肝脏发生病变时，肝细胞合成蛋白质的功能减退，血浆中蛋白质即会发生质和量的变化。临床上用各种方法检... 查看更多>

人白介素10(IL10)ELISA试剂盒使用说明书分析方法生物在线 Labon...

2021-09-23

US Biomax品牌介绍产品分类/代理商试剂采购查看更多>

糖化血清蛋白的临床意义

2021-09-08

糖化血清蛋白（GSP）与糖化血红蛋白相似，反映的是过去1～3周平均血糖浓度，由于所有糖化血清蛋白都是果糖胺，故认为测定果糖胺主要是测定糖化血清蛋白。由于血清蛋白合成比血红蛋白快（清蛋白半衰期约20天），所以糖化血清蛋白的浓度反映的是近1～3周血糖的情况，在反映控制血糖效果上比糖... 查看更多>

蛋白质的浓缩方法

2021-09-20

丙酮沉淀法；免疫沉淀法；三氯醋酸沉淀法；硫酸铵沉淀法；（低温）有机溶剂沉淀法；聚乙二醇沉淀法；超滤法；透析法；离子交换层析和冷冻干燥法……1，丙酮沉淀法；三氯醋酸沉淀法试验要求的仪器简单，但是常常导致蛋白质变性。2，免疫沉淀法：得有特异性抗体！3，硫酸铵沉淀法：利用高浓度查看更多>

马铃薯无机焦磷酸酶基因cDNA克隆及其反义植物表达载体构建【...

2021-08-28

美国monobind试剂原装进口，厂家直采查看更多>

BioWORLD 品牌介绍及代理商/经销商名单品牌专区

2021-08-30

美国ImmunoBioScience Corp（IBSC）公司介绍/品牌代理查看更多>

电泳和Western转印的常见问题实验方法

2021-09-15

表1 Common problems for electrophoresis and western bloting 问题可能原因建议解决方法推荐电压条件下电泳时间过长。电泳缓冲液过稀。配制新鲜缓冲液，使用1X稀释液。推荐电压条件下电流过高，热量过大。电泳缓冲液过稀。配制新鲜缓冲液，使用1X稀释液。推荐电压条件下电查看更多>

表达和纯化单克隆抗体:在一个基础研究实验室我们能做什么?

2021-09-09

制备高特异性、高效价的抗体是实验免疫学技术的基础，抗体质量的高低将直接影响试验的成败。通过不同方法制备的抗体往往与多种杂蛋白混杂在一起，为了获得成分相对单一的抗体或将抗体用于特定的用途，就需要进行抗体纯化。从成分的角度分析, 抗体分子是一类蛋白质分子，与其他蛋白质一样... 查看更多>

液氮罐招标采购

2021-09-02

SPM-Biocameltec品牌介绍产品代理查看更多>

TonkBio品牌试剂盒有哪些?全在这里了_生物技术_问答中心_问答...

2021-09-01

TonkBio品牌试剂盒有哪些？全在这里了查看更多>

Douglas个人资料电影视频全集1905电影网

2021-09-10

牛血清蛋白(V)第五组分是个什么概念？查看更多>

常见问题

蚂蚁淘所售产品均为正品吗？

蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑； Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。

下单后可以修改订单吗？

未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。

商品几天可以发货？

现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。

订单如何取消？

如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。

可以开发票吗？

本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。

如何联系商家？

蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，或拨打4000-520-616，除此之外客户可在联系我们页面找到更多的联系方式。

收到的商品少了/发错了怎么办？

同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

退换货/维修需要多长时间？

一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

商品咨询

IgG纯化的血清含有血红蛋白该怎么办生物科学学术科研...123

shenfx5212009-08-08

我所分离的血清中含有较多的血红蛋白，我是通过辛酸-硫酸铵沉淀和high-Q离子层析，在通过辛酸-硫酸铵之后的沉淀还是红色的，不知道能不能过离子交换柱，会不会堵死，或者有什么方法把血红蛋白除去，请高手指教，谢谢大家了

纯化多抗血清,蛋白胶出现怪异条带?请各路大神答疑解难? 123

zybkiller72021-07-26

55KDa与24KDa目的条带处的目的条带为什么成“小草”一样，旁边没有mark的话就没有条带，有mark就出现“小草”样的条带，请各位大神答疑解难？感激不尽

一文教你如何保存纯化后的蛋白质?123

Scomilo72021-08-11

保存方法如下：
　　1.以沉淀形式保存在高浓度的硫酸铵中。
　　2.添加50%甘油冷冻，尤其适用于酶类。
　　3.如果样品要用于生物学检测，避免使用防腐剂。在体内实验中不能添加防腐剂，而应当将样品分装成小份冷冻。
　　4.可使用无菌滤器以延长保存时间。
　　5.添加稳定剂，如甘油（5-20%）、血清白蛋白（10mg/ml）、配基（浓度取决于活性蛋白的浓度）以帮助维持生物活性。
　　6.避免反复冻融或冻干重溶解过程，这样很可能会降低生物活性。
　　7.一些冷沉淀蛋白会在4度沉淀出来，包括一些小鼠IgG3亚族的抗体，不能保存在4度冰箱中，可添加防腐剂保存在室温中。

蛋白质的分离纯化方法123

哲宇丶01962021-08-16

一.血清ǐ-球蛋白的初步提取
1. 血细胞与血清分离：
取人血液 1000ml,放置10min, 1000rpm离心20min .弃沉淀,留上清备用(沉淀为血细胞,上部为血清).
2. 乳糜粒分离：4000rpm 10°C离心10分钟,采用密度梯度离心
梯度液配置：离心管下部3/4容积加血浆,上部1/4容积加0.5MnaCl+0.3MEDTA,PH7.4 乳糜粒上浮,将乳糜粒吸出,留其余液体备用.
3. 血清蛋白分离：除去球蛋白,白蛋白及其它蛋白质.

【求助】蛋白分子量与预期不符经验共享分析测试百科123

aaeryuelan2021-08-10

用大肠杆菌表达纯化的蛋白，和人血清中这种蛋白的分子量不同，western显示血清中这种蛋白的分子量高于纯化的蛋白，请问各位这是为什么呢？如何解释呢？

【求助】纯化蛋白123

catzp2021-08-08

各位大虾，我想问春花出来的蛋白如果用来打兔子制作一抗，那么变性纯化的蛋白用不用复性？我的蛋白是用Ni柱纯化，其中含有尿素和咪唑，这些用不用除去在进行注射呢？
谢谢各位

胃泌素17偏高,胃蛋白酶原II偏高,胃蛋白酶原I/_有问必答_寻医问药网123

冬冬比1262021-08-01

我认为应该采取静脉注射的方法将该蛋白加入实验鼠中.以防止蛋白进入消化道中被消化.其他条件相同情况下再一个对照组,观察小鼠的变化.

北京科技大学论坛北科bbs123

2021-07-22

实验原理：
蛋白质在水溶液中的溶解度是由蛋白质周围亲水基团与水形成水化膜的程度，以及蛋白质分子带有电荷的情况决定的。当用中性盐加入蛋白质溶液，中性盐对水分子的亲和力大于蛋白质，于是蛋白质分子周围的水化膜层减弱乃至消失。同时，中性盐加入蛋白质溶液后，由于离子强度发生改变，蛋白质表面电荷大量被中和，更加导致蛋白溶解度降低，使蛋白质分子之间聚集而沉淀。一般33%硫酸铵为沉淀Ig-G的最适饱和度

血清球蛋白提纯实验报告 123

绝情qBS52018-01-10

血清中蛋白质按电泳法一般可分为五类：清蛋白、α1-球蛋白、α2-球蛋白、β-球蛋白和γ-球蛋白，其中γ-球蛋白含量约占16％，100 mL血清中约含1.2 g左右。不同种类的蛋白质其分子量、溶解度，以及在一定条件下带电荷的情况有所不同，可根据这些性质的差别，分离及提纯蛋白质。首先利用清蛋白和球蛋白在高浓度中性盐溶液中(常用硫酸铵)溶解度的差异而进行沉淀分离，此为盐析法。半饱和硫酸铵溶液可使球蛋白沉淀析出，清蛋白则仍溶解在溶液中，经离心分离，沉淀部分即为含有γ-球蛋白的粗制品。用盐析法分离而得的蛋白质中含有大量的中性盐，会妨碍蛋白质进一步纯化，因此首先必须去除。常用的方法有透析法、凝胶层析法等。本实验采用凝胶层析法，其目的是利用蛋白质与无机盐类之间分子量的差异。当溶液通过SephadexG-25凝胶柱时，溶液中分子直径大的蛋白质不能进入凝胶颗粒的网孔，而分子直径小的无机盐能进入凝胶颗粒的网孔之中．因此在洗脱过程中，小分子的盐会被阻滞而后洗脱出来，从而可达到去盐的目的。纯化后的γ-球蛋白可利用醋酸纤维素薄膜电泳法鉴定其纯度。

【求助】GST蛋白纯化后出现大量条带蛋白质和糖学123

zybkiller72018-01-23

CRESCENTBIO产品目录_第47页_Chemicalbook123

匈婐2021-08-15

就是血清里面的蛋白A高出了正常的比例。也就是说原本那个东西100才对的而你那个已经是110了。

白鳥健次歌曲大全_白鳥健次最新歌曲_九酷音乐123

少司命AU07PP362021-08-14

抗体纯化分了几种类型，根据用途决定选择哪种方法进行纯化：
1. 粗纯：将制备抗体的血清或是腹水，细胞上清，直接用盐析法进行处理，这样可以将这些物质里面的其他杂质去掉，获得蛋白的成分，但是由于是粗纯，里面会混有大量的其他蛋白，这样获得的抗体，纯度较低，用于实验中背景比较高。
2.通用型纯化：用抗体结合蛋白Protein A，Protein G或者Protein L。因为不同来源的抗体和这些抗体结合蛋白的结合能力不同，所以需要根据抗体来源选择使用哪种抗体将诶和蛋白最好。对于有一些单链抗体，则多半使用protein L来进行纯化。经过抗体结合蛋白的亲和纯化后，溶液中基本只保留了抗体的成分，其他蛋白都去掉了，抗体纯度可以比较高。相对来说，这种方法是大规模抗体制备中，用得最多的纯化方法，很多抗体公司都采用这种方法来对抗体进行纯化。
3.特异型纯化：但是有些抗体，需要纯度特别高，特异性特别好，就不能简单采用上述两种方法进行纯化了。必须要通过将抗原固定制备成特异的亲和纯化柱，再纯化抗体。这个时候得到的就全是针对一种抗原的抗体了，特异性最好。当然，由于牵涉到抗原固定等操作，成本相应是最高的。