
Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | Epinephrine-Norepinephrine ELISA kit - Research |
Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only. |
Format | 3 x 96-well plate |
Samples | Any sample |
Sample volume | 1 - 750 µl |
Cross specie-reactivity | React with all species |
Standard range | Epinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml |
Sensitivity | Epinephrine, Dopamine: 10 pg/ml Norepinephrine: 4 pg/ml |
Specificity | No significant cross-reactivity was observed with Epinephrine, Norepinephrine and Dopamine analogs such as Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
Assay time | Sample preparation 4 hours and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
ELISA | Dopamine, Epinephrine, Norepinephrine antisera overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
- Horckmans M et al. Gene deletion of P2Y4 receptor lowers exercise capacity and reduces myocardial hypertrophy with swimming exercise. Am J Physiol Heart Circ Physiol. 2012 Oct 1;303(7):H835-43.
- Schneeberger M et al. Deletion of miRNA processing enzyme Dicer in POMC-expressing cells leads to pituitary dysfunction, neurodegeneration and development of obesity. Molecular Metabolism. 2013 Apr.
- Ohlsson C et al. Estrogen receptor-α expression in neuronal cells affects bone mass. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):983-8.
- Vida G et al. α7-cholinergic receptor mediates vagal induction of splenic norepinephrine. J Immunol. 2011 Apr 1;186(7):4340-6.
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我理解就是一个是包裹,一个是镶嵌
结合物垫在层析时荧光微球在T线前面凝集的现象怎么解决呢,结合物垫增加了吐温和糖的含量还是有这种现象的
1、该制剂为肌肉注射;
2、制备该微球过程中用到了二氯甲烷、乙醇、正庚烷、二甲基硅油。国家药典有机溶剂残留规定:二氯甲烷残留限度为0.06%;乙醇残留限度为0.5%;正庚烷残留限度0.5%;二甲基硅油药典中还为収载。目前自制微球的二氯甲烷残留约0.2%左右,正庚烷残留1.0%左右,乙醇残留0.3%左右,二甲基硅油暂未检测。很明显:二氯甲烷和正庚烷超标。后来测了一下原研制剂的有机溶剂残留:二氯甲烷残留约0.1%左右,正庚烷残留1.5%左右,乙醇残留0.2%左右。本人也制剂新手,请各位大神帮帮忙,积极发言。我这个长效缓释PLGA微球制剂中有机溶剂残留限应该定多少合适,我后期优化工艺时对上述有机残留应该控制到多少一下合适。

