
Product Name | mCRAMP, mouseGLLRKGGEKIGEKLKKIGQKIKNFFQKLVPQPEQ |
Size | 1 mg |
Catalog # | AS-61305 |
US$ | $283 |
Purity | % Peak Area By HPLC ≥ 95% |
This cathelicidin-related antimicrobial peptide (mCRAMP) is the sole murine cathelicidin. mCRAMP expression in the intestinal tract is restricted to surface epithelial cells in the colon. mCRAMP shows antimicrobial activity against the murine enteric pathogen Citrobacter rodentium and destroys skin Candida albicans. | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
References | Limura, M. et al. J. Immunol. 174, 4901 (2005); Lopez-Garcia, B. et al. J. Invest. Dermatol. 125, 108 (2005). |
Molecular Weight | 3878.7 |
GLLRKGGEKIGEKLKKIGQKIKNFFQKLVPQPEQ | |
Sequence(Three-Letter Code) | H - Gly - Leu - Leu - Arg - Lys - Gly - Gly - Glu - Lys - Ile - Gly - Glu - Lys - Leu - Lys - Lys - Ile - Gly - Gln - Lys - Ile - Lys - Asn - Phe - Phe - Gln - Lys - Leu - Val - Pro - Gln - Pro - Glu - Gln - OH |
Product Citations | Sakoulas, G. et al. (2014). Nafcillin enhances innate immune-mediated killing of methicillin-resistant Staphylococcus aureus. J Mol Med 92, 139. Subramanian, H. et al. (2013). β-Defensins activate human mast cells via Mas-Related Gene X2. J Immunol 191, 345. doi:10.4049/jimmunol.1300023.Kulkarni, MM. et al. (2011). Mammalian antimicrobial peptide influences control of cutaneous Leishmania infection.Cell Microbiol 13, 913. doi: 10.1111/j.1462-5822.2011.01589.x.Gregorio, J. et al. (2010). Plasmacytoid dendritic cells sense skin injury and promote wound healing through type I interferons. J Exp Med 207, 2921.Gable, JE. et al. (2009). Fluorescence and UV resonance Raman study of peptide−vVesicle interactions of human cCathelicidin LL-37 and its F6W and F17W mutants Biochem 48, 11264. doi: 10.1021/bi900996q.Schlamadinger, DE. et al. (2009). Toxins and antimicrobial peptides: interactions with membranes.SPIE Proceedings 7397 doi: 10.1117/12.827439.Gryllos, I. et al. (2008). Induction of group A Streptococcus virulence by a human antimicrobial peptide. PNAS 105, 16755. |
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我理解就是一个是包裹,一个是镶嵌
结合物垫在层析时荧光微球在T线前面凝集的现象怎么解决呢,结合物垫增加了吐温和糖的含量还是有这种现象的
1、该制剂为肌肉注射;
2、制备该微球过程中用到了二氯甲烷、乙醇、正庚烷、二甲基硅油。国家药典有机溶剂残留规定:二氯甲烷残留限度为0.06%;乙醇残留限度为0.5%;正庚烷残留限度0.5%;二甲基硅油药典中还为収载。目前自制微球的二氯甲烷残留约0.2%左右,正庚烷残留1.0%左右,乙醇残留0.3%左右,二甲基硅油暂未检测。很明显:二氯甲烷和正庚烷超标。后来测了一下原研制剂的有机溶剂残留:二氯甲烷残留约0.1%左右,正庚烷残留1.5%左右,乙醇残留0.2%左右。本人也制剂新手,请各位大神帮帮忙,积极发言。我这个长效缓释PLGA微球制剂中有机溶剂残留限应该定多少合适,我后期优化工艺时对上述有机残留应该控制到多少一下合适。

