Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 105548 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV1 | 105548-AAV1 | Virus (100 µL at titer ≥ 1×10¹3 vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV5 | 105548-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
AAV8 | 105548-AAV8 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameTurboRFP
- PromoterCMV
Cloning Information
- Cloning methodUnknown
Resource Information
- Supplemental Documents
- pENN.AAV.CMV.TurboRFP.WPRE.RBG (P2177).gb
- Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs
- Industry Terms
- Not Available to Industry
Depositor Comments
Penn Vector Core number p2177
Information for AAV1 (Catalog # 105548-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV5 (Catalog # 105548-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV8 (Catalog # 105548-AAV8)(Back to top)
Purpose
Ready-to-use AAV8 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA.
CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV8
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneTurboRFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。
我们公司也是做磁珠的
还不错
不妨可以联系下我们
以常用于电源滤波的 HH-1H3216-500为例,其型号各字段含义依次为:HH 是其一个系列,主要用于电源滤波,用于信号线是HB系列;1 表示一个组件封装了一个磁珠,若为4则是并排封装四个的;
H 表示组成物质,H、C、M为中频应用(50-200MHz),T低频应用50MHz),S高频应用(200MHz);
3216 封装尺寸,长3.2mm,宽1.6mm,即1206封装;
500 阻抗(一般为100MHz时),50 ohm。其产品参数主要有三项:
阻抗[Z]@100MHz (ohm) : Typical 50, Minimum 37;直流电阻 DC esistance (m ohm): Maximum 20;
额定电流 Rated Current (mA): 2500。
磁珠如何用万用表测量:
用万用表的欧姆档,测量磁珠引脚两端,通(电阻值小于1欧)即为完好。
电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。
电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。