Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-315
Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SUMO Activating Enzyme E1 (SAE1/UBA2)
Small Ubiquitin-like Modifier (SUMO) Activating Enzyme Subunit 1 (SAE1) is the highly conserved human ortholog of yeast Ubiquitin-activating enzyme E1-like (UBA2) (1). These SUMO-activating (E1) enzymes are critical for the enzymatic attachment of SUMO molecules to a target protein by a post-translational modification process termed SUMOylation (2-4). The ATP-dependent E1 enzyme charges the SUMO by forming a high-energy thiol ester intermediate which is transferred to the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (5,6). The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9. UBE2I/Ubc9 is the only known E2 that is able to mediate the conjugation of SUMO to lysine residues on a variety of cellular targets, usually in the absence of a Ubiquitin ligase (E3). Although UBE2I/Ubc9 can directly recognize and modify lysine residues contained in a SUMOylation motif, E3-like factors most likely facilitate the SUMOylation of specific substrates.
Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/UBA2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high energy thiolester bond with the cysteine residue ofUBA2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.
- Johnson, E.S. et al. (1997) EMBO J. 16:5509.
- Desterro, J.M. et al. (1997) FEBs. Lett. 417:297.
- Bettermann, K. et al. (2012) Cancer Lett. 316:113.
- Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
- Okuma, T. et al. (1999) Biochem. Biophys. Res.Commun. 254:693.
- Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
Citations for Recombinant Human SUMO E1 (SAE1/UBA2) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
6Citations: Showing 1 - 6Filter your results:
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- Mechanism of Adenovirus E4-ORF3-Mediated SUMO ModificationsAuthors: SY Sohn, P HearingMBio, 2019;10(1):.Species: HumanSample Types: Recombinant ProteinApplications: Enzyme Assay
- TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFbAuthors: X Ma, T Yang, Y Luo, L Wu, Y Jiang, Z Song, T Pan, B Liu, G Liu, J Liu, F Yu, Z He, W Zhang, J Yang, L Liang, Y Guan, X Zhang, L Li, W Cai, X Tang, S Gao, K Deng, H ZhangElife, 2019;8(0):.Species: HumanSample Types: Recombinant ProteinApplications: SUMOylation Assay
- Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegansAuthors: AC Davis-Roca, NS Divekar, RK Ng, SM WignallPLoS Genet., 2018;14(9):e1007626.Species: C. elegansSample Types: Cell LysatesApplications: Bioassay
- HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cellsAuthors: WF Wang, L Yan, Z Liu, LX Liu, J Lin, ZY Liu, XP Chen, W Zhang, ZZ Xu, T Shi, JM Li, YL Zhao, G Meng, Y Xia, JY Li, J ZhuNat Commun, 2017;8(1):363.Applications: Bioassay
- Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J LuoBMC Mol. Biol., 2017;18(1):23.Species: N/ASample Types: ProteinApplications: Bioassay
- The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongationProc Natl Acad Sci USA, 2016;113(24):6725-30.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
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电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。
2、检测电感量,电阻及阻抗,那要看客户的要求。
3、磁珠是有电感值要求的。 电感值的高低 代表了磁导率的高低。电感值越大,阻抗越高。(前提是磁芯的磁导率高频衰减小,阻抗就高)
电阻 电子元器件(resistor):是一种纯阻抗,对任何频率的体现的阻值都一样,不随频率变化而变化。
我们公司也是做磁珠的
还不错
不妨可以联系下我们
以常用于电源滤波的 HH-1H3216-500为例,其型号各字段含义依次为:HH 是其一个系列,主要用于电源滤波,用于信号线是HB系列;1 表示一个组件封装了一个磁珠,若为4则是并排封装四个的;
H 表示组成物质,H、C、M为中频应用(50-200MHz),T低频应用50MHz),S高频应用(200MHz);
3216 封装尺寸,长3.2mm,宽1.6mm,即1206封装;
500 阻抗(一般为100MHz时),50 ohm。其产品参数主要有三项:
阻抗[Z]@100MHz (ohm) : Typical 50, Minimum 37;直流电阻 DC esistance (m ohm): Maximum 20;
额定电流 Rated Current (mA): 2500。