Recombinant Human Octa-Ub/Ub8 WT Chains (K63-linked), CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-318B
Formulation | 1 mg/ml (15 μM) in sterile, deionized water |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Octa-Ubiquitin
With a predicted molecular weight of 68 kDa, octa-Ubiquitin chains are composed of eight Ubiquitin monomers, which are covalently linked through isopeptide bonds that typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through each of the lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).
Linkage specific octa-Ub can be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases, the proteasome or other proteins that contain Ubiquitin-associated domains (UBAs) or Ubiquitin-interacting motifs (UIMs). This product is formed with wild-type Ubiquitin and linkage-specific enzymes. It has been shown that the rate of Ubiquitin-substrate conjugate degradation is related to poly-Ubiquitin chain length. Tetra-Ubiquitin is the minimal unit required for recognition by the proteasome, and longer chains probably have enhanced binding to proteasomal subunits and may be more resistant to disassembly by a proteasome-associated isopeptidases.
- Scheffner, M. et al. (1995) Nature 373:81.
- Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
- Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
- Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
- Henry, A.G. et al. (2012) Dev. Cell 23:519.
- Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
- Wei, W. et al. (2004) Nature 428:194.
- Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Citation for Recombinant Human Octa-Ub/Ub8 WT Chains (K63-linked), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
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电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。
2、检测电感量,电阻及阻抗,那要看客户的要求。
3、磁珠是有电感值要求的。 电感值的高低 代表了磁导率的高低。电感值越大,阻抗越高。(前提是磁芯的磁导率高频衰减小,阻抗就高)
电阻 电子元器件(resistor):是一种纯阻抗,对任何频率的体现的阻值都一样,不随频率变化而变化。
我们公司也是做磁珠的
还不错
不妨可以联系下我们
以常用于电源滤波的 HH-1H3216-500为例,其型号各字段含义依次为:HH 是其一个系列,主要用于电源滤波,用于信号线是HB系列;1 表示一个组件封装了一个磁珠,若为4则是并排封装四个的;
H 表示组成物质,H、C、M为中频应用(50-200MHz),T低频应用50MHz),S高频应用(200MHz);
3216 封装尺寸,长3.2mm,宽1.6mm,即1206封装;
500 阻抗(一般为100MHz时),50 ohm。其产品参数主要有三项:
阻抗[Z]@100MHz (ohm) : Typical 50, Minimum 37;直流电阻 DC esistance (m ohm): Maximum 20;
额定电流 Rated Current (mA): 2500。