easYmer HLA-B*41:01 MHC Tetramers Kit
easYmer HLA-B*41:01 MHC Tetramers Developed and Manufactured by immunAware
Materials Included:
The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:
- easYmer: HLA-B*41:01 – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
- Folding Buffer: Tris/Maleate pH7
- Peptide: YEHYFVFAA, a stable HLA-B*41:01 binder. Positive control for evaluation analysis of peptide-HLA folding.
For Research Use Only
Available Sizes:
- 20 Tests (Sample-size)
- 50 Tests (Standard Size)
- 150 Tests
- 500 Tests
Key Benefits of easYmer® MHC Tetramers
- Ready-to-use
- One step loading
- Completely flexible and customizable*
- Biotinylated
- No special equipment needed
- Long shelf-life
*For Custom Tetramer Production, please Contact Us for more information
Assay Principle
This easYmer HLA-B*41:01 MHC Tetramers protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.
Products Related to easYmer HLA-B*41:01 MHC Tetramers
easYmer HLA-B*46:01 MHC Tetramers Kit
easYmer HLA-B*55:01 MHC Tetramers Kit
easYmer HLA-B*57:02 MHC Tetramers Kit
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
（1）水浴式PCR仪：变温快、时间准、温度均一，但体积大，自动化程度不高。
（2）变温金属块式PCR仪：通过半导体加热和冷却，并由微机控制恒温和冷热处理过程。装置比较牢固耐用，温度变换平稳，有利于保持Taq DNA聚合酶的活性。
（3）变温气流式PCR仪：以空气作为热传播媒介，由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低；安全程度高。微机配上软件，可灵活编程。
求Hema9600基因扩增仪用户手册，实验室有这个仪器，官网（http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154）找不到说明书。
308C是16孔道的，单次反应可检测1-14项不同指标。
要做滚环扩增，实验室没有荧光光谱仪，可以用荧光定量PCR仪代替吗？
