Description | This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. |
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SKU | K012 |
System | Lentiviral Vector |
Vector Map | pLenti-EF1a-dCas9-SV40-Puro |
Unit quantity | 10 µg |
Promoter | EF1a |
Cas Type | Null Mutant |
Cas Origin | spCas9 |
Cas9 Expression | Constitutive Cas9 |
Shipping Conditions | Shipped at ambient temperature |
Bacterial Selection | Kanamycin |
Format | Vector |
Appearance | Liquid |
Quality Control | Restriction Enzyme Digest and Sequencing |
Shelf Life | 1 year (when stored at -20°C or below) |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
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308C是16孔道的,单次反应可检测1-14项不同指标。
(1)水浴式PCR仪:变温快、时间准、温度均一,但体积大,自动化程度不高。
(2)变温金属块式PCR仪:通过半导体加热和冷却,并由微机控制恒温和冷热处理过程。装置比较牢固耐用,温度变换平稳,有利于保持Taq DNA聚合酶的活性。
(3)变温气流式PCR仪:以空气作为热传播媒介,由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低;安全程度高。微机配上软件,可灵活编程。
要做滚环扩增,实验室没有荧光光谱仪,可以用荧光定量PCR仪代替吗?
求Hema9600基因扩增仪用户手册,实验室有这个仪器,官网(http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154)找不到说明书。