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Product Specifications:
Item# 1131-B: Biotin Murine Anti-gp120 HIV-1 YU2 mAb IgG
Mass/vial: Approximately 50µg IgG
Volume: 50µl
Diluent: PBS
Stabilizer: 2.5% BSA
Preservative: 0.02% Azide
Storage: 4°C
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 6 months at 4°C
Minimum Dilution: 1 : 5000
Applications: ELISA, Westerns, FACS, Immunohistopcytology.
Description: Biotin conjugated Murine anti-gp120 (HIV-1) YU2 M Tropic mAb
Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 gp120 YU2 M Tropic and HIV-1 MN ( Dual Tropic ), but not to HIV-1 ADA M Tropic, in Dot Blot assays, ELISA and Western ELISA.
Biological Activity: None
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg gp120 range with 0.1-0.5µg/ml conjugate.
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请各位高手指点一下小弟吧！
转谷氨酰胺酶(TG)、过氧化酶(POD)、多酚氧化酶(PPO)交联
戊二醛，便宜，但有毒性
EDC，无毒性，但较贵
SMCC ,DSS,DST MBS,SPDP
我最近在做磁珠与蛋白的偶联，想利用测上清液中剩余的蛋白量来检测偶联率，但是我发现交联剂对BCA法检测上清蛋白含量有显然影响，不知道有没有师兄师姐做过，最后是怎么解决偶联率检测的问题的。求教，谢谢！
从交联剂的配制，以及交联反应体系，交联后样品是否沸水煮10min,最后SDS-PAGE检测。
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