SoniBeast-Jr
The SoniBeast-Jr is a hand-held, cordless, variable-speed version of our high-energy MiniBeadbeater/Sonibeast series of bead-mill cell disruptors. While the SONIBEAST-jr is targeted for processing samples in the field or locations remote from the laboratory, it is also an affordable choice for processing a limited number of samples (<20/day) in the laboratory. Manually operated with a slide switch on the body of the SoniBeast jr's motor housing, the vial tray holds two 2 ml screw-top polypropylene microvials or one 7 ml screw-top polypropylene vial.
Our Price : $0.00
The SoniBeast-jr comes with a spare Lithium-ion battery (two total) and a smart charging station powered by 120 VAC electrical input. Beads and vials, which will vary with the application, should be ordered separately. Technical and ordering information can be found at Lysis Beads and Vials or by contacting us at Ph.918-336-3363 or info@biospec.com.
The SoniBeast-Jr is a cordless, handheld version of our MiniBeadbeater/Sonibeast series of bead-mill cell disrupters.
- Processes one or two 2 ml screw cap microvials or one 7 ml screw cap tube.
- Manually operated by an on/off slide switch on the body of the SoniBeast-Jr's motor housing.
- Variable speed control
- 10,000 to 18,000 oscillations per minute
- Cell disruption times are typically 2 minutes.
- Powered with a 1.3 amp/hour lithium-ion battery
- Includes charging station powered by 110-120VAC wall socket
- Weight: 2.1 pounds
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SoniBeast - Small Sample Cell Disruptor
Quicker, Simpler, Less Consumables, Smaller Footprint, Affordable
- Extremely Fast Cell Lysis: <20 sec
- Perfect for Small Samples
- 12 Vial Capacity
- Simple One Button Operation
The SoniBeast™ is the newest addition to our stable of high energy BeadBeater cell disruptors. Small samples of tissue or cells are completely disrupted in seconds rather than minutes. It processes up to twelve 0.6 ml locking-cap PCR microtubes, each containing 1-100 mg of tissue, or three 2 ml microvials each containing 10-400 mg of tissue. The SoniBeast is designed for wet milling in water-based media. Common applications are the isolation of intracellular protein and nucleic acids.
A pack of polypropylene 0.6 ml locking cap PCR tubes is included with the SoniBeast. Additional Vials should be ordered separately. Beads size and composition vary with the kind of sample being disrupted. For guidance on choosing beads see Lysis Beads or call us at 918-336-3363 for advice.
If you plan to power the SoniBeast with 220-240 Volt AC rather than 110-120 Volt AC please select product number "715-12-EUR".
A hand-held, cordless, smaller capacity version of the Sonibeast is available (product number "SBJ319"). Designed for samples taken in the field or other locations distant from a diagnostic laboratory, it is powered by a rechargeable lithium battery and is capable of complete homogenization of plant or animal tissues in either two 2 ml microvials or one 7 ml vial.
Read more >Price: $1,584.00
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上面较高的是样本曲线，下面较低的是未加样本（茎环引物和引物、探针都加入)
引物，又名引子。是一小段单链DNA或RNA，作为DNA复制的起始点，在核酸合成反应时，作为每个多核苷酸链进行延伸的出发点而起作用的多核苷酸链，之所以需要引物是因为在DNA合成中DNA聚合酶仅仅可以把新的核苷酸加到已有的DNA链上。
PCR即聚合酶链式反应是一种用于放大扩增特定的DNA片段的分子生物学技术，它可看作是生物体外的特殊DNA复制，PCR的最大特点，是能将微量的DNA大幅增加。
其原理为：
DNA的半保留复制是生物进化和传代的重要途径。双链DNA在多种酶的作用下可以变性解旋成单链，在DNA聚合酶的参与下，根据碱基互补配对原则复制成同样的两分子拷贝。在实验中发现，DNA在高温时也可以发生变性解链，当温度降低后又可以复性成为双链。因此，通过温度变化控制DNA的变性和复性，加入设计引物，DNA聚合酶、dNTP就可以完成特定基因的体外复制。
但是，DNA聚合酶在高温时会失活，因此，每次循环都得加入新的DNA聚合酶，不仅操作烦琐，而且价格昂贵，制约了PCR技术的应用和发展。
耐热DNA聚合酶--Taq酶的发现对于PCR的应用有里程碑的意义，该酶可以耐受90℃以上的高温而不失活，不需要每个循环加酶，使PCR技术变得非常简捷、同时也大大降低了成本，PCR技术得以大量应用，并逐步应用于临床。
PCR技术的基本原理类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物。PCR由变性--退火--延伸三个基本反应步骤构成：①模板DNA的变性：模板DNA经加热至93℃左右一定时间后，使模板DNA双链或经PCR扩增形成的双链DNA解离，使之成为单链，以便它与引物结合，为下轮反应作准备；②模板DNA与引物的退火（复性）：模板DNA经加热变性成单链后，温度降至55℃左右，引物与模板DNA单链的互补序列配对结合；③引物的延伸：DNA模板--引物结合物在72℃、DNA聚合酶（如TaqDNA聚合酶）的作用下，以dNTP为反应原料，靶序列为模板，按碱基互补配对与半保留复制原理，合成一条新的与模板DNA链互补的半保留复制链，重复循环变性--退火--延伸三过程就可获得更多的“半保留复制链”，而且这种新链又可成为下次循环的模板。每完成一个循环需2～4分钟，2～3小时就能将待扩目的基因扩增放大几百万倍。
