BiologicalActivity
Mimicofgreenfluorescentprotein(GFP)fluorophoreforimagingRNAinlivingcells.FluorescenceactivatedbybindingtoSpinach4orBroccoliaptamers.Exhibitspeakexcitationmaximaof482nmandpeakfluorescenceemissionof505nmwhenboundtoSpinach4,enablingimagingwithGFPfiltercubes.ExhibitshigherspecificfluorescenceandlowerbackgroundfluorescencewhenboundtoSpinach4comparedwithSpinach4-DFHBI.
TechnicalData
| M.Wt | 320.21 |
| Formula | C13H9F5N2O2 |
| Storage | Storeat-20°C |
| Purity | ≥98%(HPLC) |
| CASNumber | 1539318-36-9 |
| PubChemID | 101889712 |
| InChIKey | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| Smiles | O=C1N(CC(F)(F)F)C(C)=NC1=C/C2=CC(F)=C(O)C(F)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.02 | 100 | |
| ethanol | 16.01 | 50 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight320.21.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 3.12mL | 15.61mL | 31.23mL |
| 5mM | 0.62mL | 3.12mL | 6.25mL |
| 10mM | 0.31mL | 1.56mL | 3.12mL |
| 50mM | 0.06mL | 0.31mL | 0.62mL |
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