
Mouseprimaryantibodiesonrattissuesareadvantageousbecausemousesecondarydetectionsystemscanbeusedwithminimumcross-reactivitytoendogenousratIgG.BiocareMedicalhasdevelopedaMouse-on-RathorserADIshperoxidase(HRP)polymerdetectionsystemwithminimalcross-reactivitytorattissues.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsandcellculturepreparations.BiocareMedicalhasnovelproductstohelpeliminatepersistentratIgG.XRFactorisaverypotentblockerforeliminatingendogenousratIgG.Byadding1-2drops(1dropequals33µl)oftheXRFactorto2.5mloftheMouse-on-RatPolymer,endogenousratIgGwillbedramaticallyreducedand/orcompletelyeliminated.
ebiomall.com






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上面较高的是样本曲线,下面较低的是未加样本(茎环引物和引物、探针都加入)
引物,又名引子。是一小段单链DNA或RNA,作为DNA复制的起始点,在核酸合成反应时,作为每个多核苷酸链进行延伸的出发点而起作用的多核苷酸链,之所以需要引物是因为在DNA合成中DNA聚合酶仅仅可以把新的核苷酸加到已有的DNA链上。
引物设计略有不一样,但都可用Primer5设计。
http://medgen.ugent.be/rtprimerdb/

