Rabbit-on-RodentAP-Polymerhasbeendevelopedforusewithrabbitmonoclonal/polyclonalantibodiesonmouseandrattissues.Rabbitprimaryantibodiesonmouseandrattissuesareadvantageousbecauserabbitsecondarydetectionsystemscanbeusedwithminimumcross-reactivitytoendogenousmouseandratIgG.AdvantagesofBiocareMedical’spolymertechnologyareincreasedsensitivity,reductionofIHCstepsandeliminationofendogenousbiotin.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsorcellcultures.Biocare’sRabbit-on-RodentAP-Polymerisidealforsinglestainingandmayalsobeusedindoubleandtriplestainingtechniques.Insometissuessuchasspleen,endogenousmouseorratIgGmaybedifficulttoeliminate.BiocareMedicalhasdevelopedanantigenretrievalsolution(RodentDecloaker)thathelpsreduceoreliminateendogenousIgGandnon-specificbackgroundstainingwhilesimultaneously,performingantigenretrieval.Temperaturedependentprotocolsat80ºC,95ºCand125ºCcanbeperformedusingBiocare’sDecloakingChamber.BiocareMedicalhasadditionalproductstohelpeliminatepersistentratandmouseIgG.TheXMandXRFactorsaretwopotentblockersforeliminatingendogenousmouseorratIgG.Byadding1-2drops(1dropequals33µl)oftheXMorXRFactorto2.5mlofRabbit-on-RodentAP-Polymerdetection,endogenousmouseorratIgGwillbereduceddramaticallyorcompletelyeliminated.InadditionRodentBlockM(mousetissue)andRodentBlockR(rattissue)blockfornon-specificbackgroundstainingandreducestainingduetoendogenousmouseorratIgG.RodentBlockMorRisappliedtothetissuepriortotheprimaryantibodyfor15to30minutes.
ebiomall.com
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上游引物：5′-GAATGTGTGTACTGCAAGCA-3′
下游引物：5′-GTTGTATTGCTGTTCTAATGTTGT-3′
Taqman探针：5′-FAM-CAGCATATGGATTCCCATCTC-TAMRA-3′
扩增的产物长度是：195bp(nt188-382)
HPV16全长序列：贴在附件中
磕头跪谢！！！
HPV16.txt(9.91k)
http://medgen.ugent.be/rtprimerdb/
