
Biocare’sPromARKTMseriesintroducesa1-stephorserADIshperoxidase(HRP)polymerdetectionsystemforIHCofratprimaryantibodiesonmousetissues,withminimalcross-reactivity.RatHRP-Polymer,1-Step(Mouseadsorbed)offerstheconvenienceandsensitivityofaone-steppolymerdetectionsystemthatisbiotinandavidinfree.Itisspecificallyformulatedtoimprovespecificityandreducebackgroundstainingonmousetissues.TheeliminationofmouseIgGcanbeapersistentprobleminachievingoptimalstainingofmousetissues.Inadditiontothe1-stepRatHRP-Polymer(Mouseadsorbed)detection,Biocareoffersreagentsthatmayfurtherreduceunwantedbackgroundstaining.AddingXMFactordirectlytoRatHRP-PolymermayfurtherreducebackgroundstainingduetoendogenousmouseIgG.RodentBlockMmayalsobeusedtoreducenonspecificbackgroundstaining(seeindividualdatasheets).BiocarehasalsodevelopedRodentDecloaker,anantigenretrievalsolution,whichisspecificallyformulatedtoreduceand/oreliminatenon-specificbackgroundstainingduetoendogenousmouseandratIgG.TemperaturedependentantigenretrievalprotocolscanbeperformedusingBiocare’sDecloakingChamber(pressurecooker).
ebiomall.com






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设计方法:通用茎环后端加miRNA后面6个碱基的反向互补序列为反转录引物,正向引物为miRNA去除后面6个碱基的序列,反向引物在茎环上。
反转录茎环通用序列:
GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGAC
miRNA序列:
>mmu-miR-99b-5p
MIMAT0000132 Mus musculus miR-99b-5p
CACCCGUAGAACCGACCUUGCG
mmu-miR-99b-5p反转录颈环引物为:
GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCGCAAG
定量PCR反向引物为:TGTCGTGGAGTCGGC
正向引物为:CACCCGTAGAACCGAC
http://medgen.ugent.be/rtprimerdb/

