1Putmousedown2WashskinbydippingmiceintoETOH3Removetumorandputinto5mlPBScontainingFungizoneandPenstrep4Swirlaroundtorinsetumorandpourontoasterileplate5RemovetumorfromPBSplaceonsterileplateorlid6Removeanyskin-rinseasecondtimeifneeded7Chopintofinepieceswithscalpelblades8Transferusingbladeinto2mlH14mediacontainingFungizoneandPenstrep9Spindown800rpm10Removemedialeaving~300ul11Add2ml.25%trypsinandplaceonrockerfor5min12Spin800rpmfor5min13ResUSPendinDMEM/F12with10%FCS14Spin800rpmfor5min15ResuspendinH14mediacontainingFungizoneandPenstrep16Plateontovitrogencoatedflaskofplate(volumeofmediaandsizeofflaskdependontumorsizeIuseda25cm2flask) 17changemediathenextdayandkeepcloseeyeoutforcontamination
