Product Overview
Background
Plasminogen Activator Inhibitor-1 (PAI-1) is a glycoprotein and member of the serine protease inhibitor (serpin) superfamily. PAI-1 is the primary inhibitor of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). This inhibition exhibits antiproteolytic properties that can lead to myocardial infarction, thromboembolic disease with elevated levels of PAI-1. Additionally, PAI-1 is thought to play a role in the function of tissue remodeling and tumor metastasis.
Assay Principle
This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of PAI-1 levels in biological fluid. This PAI-1 test kit operates on the basis of sandwich ELISA where active PAI-1 complexes with uPA and is quantified with the use of an HRP labeled secondary antibody. The functional or active PAI-1 will bind to the uPA coated on the well of the microtiter plate. The latent and complexed forms of PAI-1 will not bind and are discarded at a later washing step. Next, a PAI-1 primary antibody is added to the wells, binding to the captured PAI-1 on the microtiter plate. HRP conjugated secondary antibody is then added for detection of the active PAI-1. Quantitative test results are obtained by the measure and comparison of the sample and standard absorbance readings.
Product Documents
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我是新手,来到丁香园看到有这么多的热心人,感到很高兴!!我也有很多问题需要大家的帮助!!实验已经作了一年了可是毫无进展,心里很是着急!!
我的课题是以外消旋的苯基乙二醇为底物,用假丝酵母催化生成手性纯的S-型苯基乙二醇,由于是老课题,所以目标是提高转化反应的底物浓度和菌体的使用批次(目前菌体使用一批后便不能再使用)。
我曾试过很多种方法,但均效果不大!我试着在转化过程中添加醛类,酮类,醇类作为辅助底物,增加菌体的使用批次。可效果不好,特别是添加了醇类后还有的起了反作用,因为我的这个转化过程中涉及到NAD和NADPH的再生。(其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮,再将酮还原为醇,经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了)
我还试过用固定化的方法,海藻酸钙包埋法,可是这样底物浓度就更低了!!
我现在不知道下一步该如何做了,很着急,请大家帮帮忙。谢谢了!!谢谢
反应底物——葡萄糖
反应产物——ATP、乙醇、CO2或ATP、乳酸
有氧呼吸:
反应底物——葡萄糖
反应产物——ATP、CO2、H2O
ELISA实验,操作的时候,加入底物,溶液变成蓝色,孵育一段时间后,加入终止液,变成黄色,测吸光值。然后,当天忘记扔板子了,第二天想到去扔的时候,所有孔都变成无色透明的了!!!为什么会褪色???是试剂有问题?还是因为室温太高环境影响?如果是试剂问题那我之前做的结果还能信么?

