ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
- Product No.KSA092Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.62-1000pg/mL
- Applicable Sensitivity5.5pg/mL.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 842 For more details, please contact local distributors!US$ 1404 For more details, please contact local distributors!US$ 2527 For more details, please contact local distributors!US$ 4914 For more details, please contact local distributors!US$ 8424 For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA092Hu01 | Recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA092Hu01 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
| APA092Hu61 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
| EPA092Hu61 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA092Hu51 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA092Hu07 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
| PAA092Hu01 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
| PAA092Hu06 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
| MAA092Hu22 | Monoclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
| SEA092Hu | ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA092Hu | CLIA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA092Hu01 | ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGA092Hu01 | Primer Pair for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | PCR |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
向左转|向右转向左转|向右转向左转|向右转向左转|向右转
调节至最适合温度(一般是37度),合适的酸碱度时,酶的活性最高
决定酶促反应最大速度Vm的因素是什么?是同一底物,尽管加不同种酶,其Vmax都相同吗?可以画曲线图解释一下吗?可是根据米曼氏方程来看又有点矛盾,Km变大,Vmax也需变大,才可使v变大。所以题目所述到底应该怎么理解呢
底物为参与生化反应的物质,可为化学元素、分子或化合物,经酶作用可形成产物。一个生化反应的底物往往同时也是另一个化学反应的产物。
特定的底物会在特定的酶作用下,合成或分解。
酶,指具有生物催化功能的高分子物质。 在酶的催化反应体系中,反应物分子被称为底物,底物通过酶的催化转化为另一种分子。几乎所有的细胞活动进程都需要酶的参与,以提高效率。与其他非生物催化剂相似,酶通过降低化学反应的活化能来加快反应速率,大多数的酶可以将其催化的反应之速率提高上百万倍;事实上,酶是提供另一条活化能需求较低的途径,使更多反应粒子能拥有不少于活化能的动能,从而加快反应速率。酶作为催化剂,本身在反应过程中不被消耗,也不影响反应的化学平衡。酶有正催化作用也有负催化作用,不只是加快反应速率,也有减低反应速率。与其他非生物催化剂不同的是,酶具有高度的专一性,只催化特定的反应或产生特定的构型。


