
ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
- Product No.KSA092Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Reagent ContentsCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.62-1000pg/mL
- Applicable Sensitivity5.5pg/mL.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM96T*596T*1096T*2096T*5096T*100
- FOBUS$ 842 For more details, please contact local distributors!US$ 1404 For more details, please contact local distributors!US$ 2527 For more details, please contact local distributors!US$ 4914 For more details, please contact local distributors!US$ 8424 For more details, please contact local distributors!
SPECIFITY of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.2. Aspirate and wash 1 time.3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
GIVEAWAYS
ELISA / CLIA Experiment Service
INCREMENT SERVICES
CLIA Kit Customized ServiceELISA Kit Customized ServiceAssay Kit DIY Support Pack 1Assay Kit DIY Support Pack 2Standard DiluentCoating BufferDiluent Buffer for AntibodyAssay Kit DIY Support Pack 3Assay Kit DIY Support Pack 4Real Time PCR Experimental Service
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA092Hu01 | Recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA092Hu01 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
APA092Hu61 | Active Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Cell culture; Activity Assays. |
EPA092Hu61 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA092Hu51 | Eukaryotic Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA092Hu07 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu01 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
PAA092Hu06 | Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
MAA092Hu22 | Monoclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a) | WB; IHC; ICC; IP. |
SEA092Hu | ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA092Hu | CLIA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Chemiluminescent immunoassay for Antigen Detection. |
KSA092Hu01 | ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | Main materials for "Do It (ELISA Kit) Yourself". |
PGA092Hu01 | Primer Pair for Macrophage Inflammatory Protein 1 Alpha (MIP1a) | PCR |
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我是新手,来到丁香园看到有这么多的热心人,感到很高兴!!我也有很多问题需要大家的帮助!!实验已经作了一年了可是毫无进展,心里很是着急!!
我的课题是以外消旋的苯基乙二醇为底物,用假丝酵母催化生成手性纯的S-型苯基乙二醇,由于是老课题,所以目标是提高转化反应的底物浓度和菌体的使用批次(目前菌体使用一批后便不能再使用)。
我曾试过很多种方法,但均效果不大!我试着在转化过程中添加醛类,酮类,醇类作为辅助底物,增加菌体的使用批次。可效果不好,特别是添加了醇类后还有的起了反作用,因为我的这个转化过程中涉及到NAD和NADPH的再生。(其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮,再将酮还原为醇,经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了)
我还试过用固定化的方法,海藻酸钙包埋法,可是这样底物浓度就更低了!!
我现在不知道下一步该如何做了,很着急,请大家帮帮忙。谢谢了!!谢谢
酶促反应速率跟底物浓度,pH,温度,酶的 浓度等因素有关,当底物浓度足够高时,酶的浓度相对较低,酶只能与一部分底物结合,导致速率有一个最大值.
ELISA实验,操作的时候,加入底物,溶液变成蓝色,孵育一段时间后,加入终止液,变成黄色,测吸光值。然后,当天忘记扔板子了,第二天想到去扔的时候,所有孔都变成无色透明的了!!!为什么会褪色???是试剂有问题?还是因为室温太高环境影响?如果是试剂问题那我之前做的结果还能信么?

