
Test Label: NATest Format: ImmunoStripCapture Reagent: PolyclonalDetection Reagent: Monoclonal
Intended Use:
This ImmunoStrip® test is intended for seed quality purposes to determine the presence or absence of eCry3.1Ab protein in transgenic corn (Event 5307, Agrisure Duracade®). This test system can be used to test individual corn seed and corn leaf or detect 1 transgenic eCry3.1Ab seed in 100 corn seeds (1.0 %).The eCry3.1Ab ImmunoStrip® has shown no cross-reaction with other transgenic proteins in corn seed and leaf including GA21 (mEPSPS), Bt11 (Cry1Ab), mCry3A, or MIR162 (Vip3A).
Includes:- ImmunoStrips®
- PBST sample buffer not included, see related products
- SEB4 extraction buffer powder not included, see related products
- User Guide
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酶促反应速率跟底物浓度,pH,温度,酶的 浓度等因素有关,当底物浓度足够高时,酶的浓度相对较低,酶只能与一部分底物结合,导致速率有一个最大值.
不同的酶Km值不同,同一种酶与不同底物反应Km值也不同,Km值可近似的反应酶与底物的亲和力大小:Km值大,表明亲和力小;Km值小,表明亲合力大。
Km最小的那个底物,就是酶的最适底物。
决定酶促反应最大速度Vm的因素是什么?是同一底物,尽管加不同种酶,其Vmax都相同吗?可以画曲线图解释一下吗?可是根据米曼氏方程来看又有点矛盾,Km变大,Vmax也需变大,才可使v变大。所以题目所述到底应该怎么理解呢

