ELISAAPSubstrate
Lumi-Phos530 isaready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,LumigenPPD.Lumi-Phos530isusefulforchemiluminescentdetectionofAPconjugatesinimmunoassaysandDNAprobeassays.Itprovidesultrasensitivedetectionofanalytescapturedonmagneticparticlesandinmicrotiterplatewells.Lumi-Phos530is10,000timesmoresensitivethancolorimetricsubstratesinsolution.
- Excellentsensitivity -lowpicogramtofemtogram
- Sustainedluminescence -withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
- Linearcalibrationcurves -theluminescenceintensityatanytimepointisadirectmeasureoftheconcentrationoftheenzyme
- Highstability andlongshelflife
ChemiluminescentEnhancement
Auniqueco-surfactantsystempromotesandenhancesenergy-transfertothefluorescentheadgroupscausingthegenerationofluminescencewithabroademissionmaximumat530nm.
