
Description:
1.INTRODUCTION
Celiacdisease,alsoknownasglutensensitiveenteropathyisprimarilyadiseaseoftheinfantorganism.ItiscausedbyahypersensitivityreactioninresponsetogliADIn,aproteinbeingpresentinmanycereals.This,nonIgEmediatedfoodallergyleadstomassivemalabsorptiondisturbancesandischaracterizedbyacompleteatrophyofthevilliandahyperplasiaofthecryptsoftheupperintestine.Accordinglypatientssufferingfromceliacdiseasemustmaintainaglutenfreedietfortherestoftheirlife.Gliadinsareproteinscontaininghighamountsoftheaminoacidsprolinandglutamine.Theseproteinsbelongtothenutritivetissueofthegrainseedsofwheat,oat,barleyandryeandareresponsIBLeforthebakingpropertiesoftheflour.DuetothepossibilitiesofthehighlyspecificandsensitiveSEROlogicaldeterminationofIgAandIgGantibodiesagainstDGPtheinvasiveproceduresofbiopsiescanbegivenup.InthepastseveralbiopsieshavebeendonewithpatientswhenceliacdiseasewassUSPected,afteraperiodofagluten-freedietandalsoafteraspecificglutenchallenge.DGPantibodiestiterhasbeenprovedtocorrelateverywellwiththemorphologicalappearanceofthemucosaoftheupperintestine.IthasbeenwelldocumentedthatDGPantibodieslevelfallveryquicklyafteraglutenfreediethasbegunandriseimmediatelyafterrestoringglutentothediet.Thustheserologicaltestrepresentsareliablemethodtomonitorpatients,andinparticularchildrenandteenagers,fortheiradherencetothegluten-freediet.
2.INTENDEDUSE
Anti-DeamidatedGliadinPeptide(DPG)IgAkitisasolidphaseenzymeimmunometricassay(ELISA)designedforthequantitativemeasurementofIgAclassantibodiesdirectedagainstdeamidatedGliadinpeptides(DGP)inhumanserumorplasma.Anti-DeamidatedGliadinPeptide(DPG)IgAisintendedforlaboratoryuseonly..
3.PRINCIPLEOFTHEASSAY
Anti-DeamidatedGliadinPeptide(DPG)IgAtestisbasedonthebindingofpresentantibodiesincalibrators,controlsorpredilutedpatientsamplesonthesynteticdeamidatedGliadinpeptides(DGP)coatedontheinnersurfaceofthewells.Aftera30minutesincubationthemicroplateiswashedwithwashbufferforremovingnon-reactiveserumcomponents.Ananti-human-IgAhorseradishperoxidaseconjugatesolutionrecognizesIgAclassantibodiesboundtotheimmobilizedantigens.Aftera30minutesincubationanyexcessenzymeconjugate,whichisnotspecificallyboundiswashedawaywithwashbuffer.AchromogenicsubstratesolutioncontainingTMBisdispensedintothewells.After15minutesofincubationthecolordevelopmentisstoppedbyaddingthestopsolution.Thesolutionscolorchangesintoyellow.TheamountofcolorisdirectlyproportionaltotheconcentrationofIgAantibodiespresentintheoriginalsample.
AdditionalInformation:
Name | AntiDeamidatedGliadinPeptide(DGP)IgA |
---|---|
RelatedProductNames | AntiDeamidatedGliadinPeptideImmunoglobulinAELISAKit,AntiDGPImmunoglobulinAELISA,AntiDGPIgAELISA,AntiDeamidatedGliadinPeptideIgAELISA,CeliacdiseaseELISA,GastroEnterologyAssay |
MolecularWeight | 0.5 |
Concentration | LotSpecific |
Applications | Enzymeimmunoassayforthequantitativedeterminationofauto-antibodiesagainstdeamidatedGliadinpeptides(DGP)inhumanserumorplasma |
Reactivity | Human |
Storage | Storeallthekitreagentsat2-8°C.Donotfreeze.ReagentsarestableuntiltheexpirationdatewhenstoredandhandledasdirectedUnusedantigencoatedmicrowellstripsshouldberesealedsecurelyinthefoilpouchcontainingdesiccantsandstoredat |
IntendedUse | ResearchUseOnly |