商品信息
联系客服
郑重提醒:
无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
GenWay/Cathepsin D Inhibitor Screening Kit/GWB-AXR114/100 assays
免费咨询热线
4000-520-616
Description:
Description:ApoptosiscanbemediatedbymechanismsotherthanthetrADItionalcaspase-mediatedcleavagecascade.Thereisgrowingrecognitionthatalternativeproteolyticenzymessuchasthelysosomalcathepsinproteasesmayinitiateorpropagateproapoptoticsignals.CathepsinsarelysosomalenzymesthatarealsousedassensitiveMarkersinvarioustoxicologicalinvestigations.TheCathepsinDActivityAssaykitisafluorescence-basedassaythatutilizesthepreferredcathepsinDsubstratesequenceGKPILFFRLK(Dnp)-D-R-NH2)labeledwithMCA.CathepsinDwillcleavethesyntheticsubstratetoreleasethequenchedfluorescentgroupMCA,whichcantheneasilybemeasuredusingafluorometerorfluorescenceplatereaderatEx/Em=328/460nm.Therelativeefficacyoftestinhibitorsarecomparedtothepositivecontrolinhibitor,PepstatinA(IC50< 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).Summary:
-Detectionmethod-Fluorescence(Ex/Em=328/460nm)
-Sampletype-PurifiedCathepsinD,RecombinantCathepsinD
-Kitsize-100assays
-Applications-AssaykittodetectputativeCathepsinDinhibitors
Kitcomponents:
-CDReactionBuffer
-CDSubstrate(1mM)
-CathepsinD,human(lyophilized)
-PepstatinA(10µMinDMSO)
FeaturesandBenefits:
-Simpleone-stepprocedure;takesonly1hours
-Fast,robustandconvenient
-Cathepsin-Dinhibitorscreeningassayisafastandrobustassaytoscreenpotentialinhibitors
-Positivecontrolinhibitor,PepstatinAisincludedinthekit
ShippingConditions:Gelpack
AdditionalInformation:
Name | CathepsinDInhibitorScreeningKit |
---|---|
RelatedProductNames | CathepsinDActivityAssayKit,Cathepsin,CathepsinD,CathepsinD,Cathepsin-D,ActiveCathepsinD,CathepsinProtein,Cathepsinactivityassay,cathepsinassay,cathepsinDassay,CathepsinDInhibitorScreeningKit,CathepsinDInhibitor |
Format | AssayKit |
Storage | -20C |
IntendedUse | ResearchUseOnly |