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GenWay/Annexin V-FITC Apoptosis Kit Plus - 100 assays/GWB-AXR150/100 assays
免费咨询热线
4000-520-616
Description:
Description:TheAnnexinV-FITCApoptosisDetectionKitPlusisbasedontheobservationthatsoonafterinitiatingapoptosis,mostcelltypestranslocatethemembranephospholipidphosphatidylserine(PS)fromtheinnerfaceoftheplasmamembranetothecellsurface.Onceonthecellsurface,PScaneasilybedetectedbystainingwithafluorescentconjugateofAnnexinV,aproteinthathasastrongnaturalaffinityforPS.Theone-stepstainingproceduretakesonly10minutes.Inaddition,theassaycanbedirectlyperformedonlivecells,withouttheneedoffixation.TheAnnexinV-FITCApoptosisDetectionKitPlusincludesannexinV-FITC,SYTOXgreendye,andbindingbuffer.TheSYTOXgreendyeisimpermeanttolivecellsandapoptoticcells,butstainsnecroticcellswithintensegreenfluorescencebybindingtocellularnucleicacids.AfterstainingacellpopulationwithannexinV-FITCandSYTOXGreendyeintheprovidedbindingbuffer,apoptoticcellsshowgreenfluorescence,deadcellsshowahigherlevelofgreenfluorescenceandlivecellsshowlittleornofluorescence.Thesepopulationscaneasilybedistinguishedusingaflowcytometrywiththe488nmlineofanargon-ionlaserforexcitation.BothannexinV-FITCandSYTOXGreendyeemitgreenfluorescencethatcanbedetectedintheFL1channel,freeingtheotherchannelsfortheadditionofotherprobesinmulti-colorlabelingexperiments.Summary:
-Detectionmethod-Flowcytometry(Ex=488nm;Em=530nm).
-Sampletype-Livecells
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100and400assays)
-Applications-AnnexinV-FITCkitincludesAnnexinV-FITCfordetectingapoptosisandalsopropidiumiodide(PI)fordetectingnecrosis.
Kitcomponents:
-AnnexinV-FITC
-SYTOXGreenDye
-BindingBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takesonly10minutes
-Fastandconvenient
-TheAnnexinVFITCPluskitincludessytoxgreendye(insteadofPI)whichstainsnecroticcellswithahigherlevelofgreenfluorescencethanFITCdoes
ShippingConditions:Gelpack
AdditionalInformation:
Name | AnnexinV-FITCApoptosisKitPlus-100assays |
---|---|
RelatedProductNames | AnnexinV-FITCApoptosisKitPlus,AnnexinV-FITCApoptosisKit,AnnexinV,AnnexinV-FITC,AnnexinVApoptosis,AnnexinVAssay,AnnexinV,AnnexinV,Annexin-V,Annexin-5,Annexin5,Annexin5 |
Format | AssayKit |
Storage | +4C |
IntendedUse | ResearchUseOnly |