Description:
ChromogenixCoatest®Heparinisa chromogenicassaykitfortheinvtirodiagnosticphotometricdeterminationofheparininhumanplasma.TheCoatest®Heparintestkitdeterminestheanti-FXaactivityofLMWheparinandUFheparin.ExcellentreagentstABIlity.Suitableforbothlargeandsmalllaboratories.
KitComposition:
Reagents, Packaging,Storage,Stability,andMicroplate/TestTubePreparation
- S-2222™,1vial.Chromogenicsubstrate(Bz-IIe-Glu-(g-OR)-Gly-Arg-pNA·HCl)15mgwithmannitoladdedasabulkingagent.Reconstitutewith20mLsterilewatertoobtainaconcentrationof1mmol/l.Thesolutionisstablefor6monthsat2-8°C.
- FactorXa,1vial.BovineFactorXa71nkat.Reconstitutewith10mLsterilewater.ThereconstitutedFactorXaisstablefor1monthat2-8°Cor6monthsat-20°Corbelow.
- Buffer,stocksolution,1vial.Tris0.5mol/l,pH8.4,10ml.Anopenedvialofstocksolutionisstablefor2monthsat2-8°C.Beforeusediluteaccordingly:1volumeofstocksolutionwith9volumesofsterilewater.
- Antithrombin,1vial.LyophilizedhumanAntithrombin,10IU.Reconstitutewith10mLsterilewatertoobtainaconcentrationof1IU/ml.ThereconstitutedAntithrombinisstablefor1monthat2-8°Cor6monthsat-20°Corbelow.
- NormalPlasma(human),4vials.Lyophiliziedplasma.Reconstitutewith1.00mLsterilewater.Thereconstitutedplasmaisstablefortwoweeksat2-8°Cor1monthat-20°Corbelow.
Whenkeptat2-8°Cthesealedreagentsarestableuntilexpirydateprintedonthelabel.Avoidcontaminationbymicroorganismsinopenedvials.
MeasurementPrinciple:
AT+ Heparin(excess) | → | [Heparin•AT] |
[Heparin• AT]+FXa(excess) | → | [Heparin• AT•FXa](excess)+FXa(residual) |
FXa(residual) | ||
S-2222™ | → | Peptide+pNA |
Heparinisanalysedasacomplexwithantithrombin(AT)presentinthesample.TheconcentrationofthiscomplexisdependentontheavailabilityofAT.InordertoobtainamoreconstantconcentrationofAT,purifiedATisaddedtothetestplasma.FXa(inexcess)isneutralizedinproportiontotheamountofheparin,whichdeterminestheamountof[Heparin•AT]complex.TheremainingamountofFXahydrolysesthechromogenicsubstrateS-2222™thusliberatingthechromophoricgroup,pNA.Thecoloristhenreadphotometricallyat405nm.
Testperkit:
- Testtube:100
- Microplate:400
- Automated:upto285
Background:
Heparinpreparationsextractedfromanimalshavebeenusedclinicallyforoverhalfacenturyasapotentanticoagulanttherapeuticforthetreatmentandpreventionofthromboticdisease.Bleedingandheparin-inducedthrombocytopeniaarethemainadversereactionsassociatedwithheparintherapy.Theseriskscanbeminimizedbyappropriatepatientmanagementandbylaboratorymonitoringusingspecificchromogenicanti-factorXaassays.Theclinicalindicationsfortheseassaysarereviewedtogetherwithabasicintroductionoftheclinicalpharmacologyofheparin.
Heparinisanaturallyoccurring,highlysulfatedpolysaccharide,characterizedbyawidemolecularweightrangeandpowerfulanticoagulantproperties.SinceitsdiscoverybyMcLeanin1916,heparinhasbecomeawidelyusedanticoagulantforthetreatmentandpreventionofthromboticdiseasesandformaintainingbloodfluidityinextracorporealdevices.Materialforclinicaluseisderivedfromporcineandbovinetissueandispreparedeitherasunfractionated(UF)heparinorasdepolymerizedlowmolecularweight(LMW)heparin.
Themaincomplicationwithheparintherapyisthatitoccasionallycauseslife-threateningbleeding.Laboratorymonitoringwithadjustmentsofdoseregimensisoneoftheoptionsavailablewhichmayimprovetheantithromboticefficacyofheparinandreducetheriskofhemorrhage.However,theidealheparintestanditsclinicalrelevanceisstillacontroversialtopic.
Readmoreaboutheparin.
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dba(oh)2nahco3alcl3nahso4
通过解答,教会我,谢谢
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2