Description:
ChromogenixS-2251™isachromogenicsubstrateforplasminandstreptokinase-activatedplasminogen.Thesubstratehasbeenusedforthedeterminationof:
- Antiplasmininplasma
- Plasminogeninplasma
Composition:
EachvialcontainschromogenicsubstrateS-2251™,25mgandmannitol60mgasabulkingagent.StABIlity:Substance:Stableuntilexpirydateifstoredat2-8°C.Avoidexposuretolight.Thesubstanceishygroscopicandshouldbestoreddry.Solution:3mmol/LinH2Oisstableforatleast6monthsat2-8°C.Contaminationbymicroorganismsmaycausehydrolysis.
Chemicalname:H-D-Valyl-L-leucyl-L-lysinep-Nitroanilinedihydrochloride
Formula:H-D-Val-Leu-Lys-pNA·2HCl
Mol.wt:551.6
PlasminogenandPlasminIUandEnzymeActivity:
Plasminogen(MW:92Kda)isthezymogenformofplasmin(83KDa).
Theactivationofplasminogenisaccomplishedbyt-PA,urokinaseorstreptokinase.
Itisnotthepurposeofthistexttodescribethemechanismsofactivationofplasminogenanditsinhibition,butitisimportanttounderlinethattheycanaffectthereactionoftheactiveenzymewithchromogenicsubstrates,caseinorfibrin.
FormoreinformationrefertoGaffney(1).
1mlofnormalplasmacontainsabout180µgor2nmolofplasminogen.Theactivityofplasminogencanbeexpressedincaseinunits(CU)e.g.forexampletoSgourisetal.(2).
Friberger(3)foundthattheamountofplasminogenin1mlofnormalplasmacorrespondsto3.8CU,asdeterminedbythechromogenicmethoddescribed(streptokinaseactivatedplasminogen).
TheChromogenixplasminogenreagentwhenactivatedwithstreptokinaseshowsanactivityof 7.3nkatchromogenicsubstrateS-2251™perCU.
In1983Friberger(4)reportedthat1µgofplasmincorrespondsto0.20nkatchromogenicsubstrateS-2251™,orto0.024CUorto0.028CTA-U.
In1975,thefirststandardforplasminwasestablished(5)andtheinternationalunitwasdefinedequivalenttoCTA-U(5-6).
Thiscontainedapurifiedhumanplasminin50%glycerol.WhentestedbyFribergeretal.(7),itshowedaboutthesameactivityastheKabipreparationintermsofµmolofsubstratehydrolysedperminuteperunitofenzyme. Sincethenthedifferentaspectsofcaseinolytic,amidolyticandfibrinolyticactivityhavebeenexploredindetailsand,thediscrepancybetweenamidolyticandfibrinolyticactivityinreferencepreparationswasunderlinedasinthecaseofthe2ndInternationalReferencePreparationofplasminin1983(8).
Thecurrent3rdInternationalStandardforplasmin,97/536wasestablishedin1998,andtheresultspresentedattheFibrinolysisSubcommitteeoftheScientificandStandardizationCommitteeoftheInternationalSocietyofThrombosisandHaemostasis(Ljubljana,Slovenia,June1998).The2ndInternationalReferencePreparationwasusedasreferencematerialandachromogenicassaywasthemethodused.ThereisnoestablishedWHOstandardforplasminogen,buttheNationalInstituteforBIOLOGicalStandardsandControl,establishedtheIstBritishReferencePreparation,78/646(9).
ItisapreparationofGlu-plasminogen,assayedbyfibrinolyticandchromogenicmethodsagainstthe2ndInternationalStandardforplasmin.Completeactivationofplasminogenwasachievedbybothurokinaseorstreptokinaseandtheactivitywascomparabletothatofthereferencepreparationofplasmin.
- GaffneyPJ.
Standardizationofplasminogenassays.Haemostasis18Suppl.1,47-60(1988). - SgourisJTetal.
Thepreparationofhumanfibrinolysin(plasmin).VoxSang5,357-376(1960). - FribergerPandKnosM.
Plasminogenactivationinnormalplasma.In:Chromogenicpeptidesubstrates,Eds,ScullyMFandKakkarVV,ChurchillLivingstone,128-140(1979). - FribergerPetal.
Syntheticpeptidesubstrateassaysandfibrinolysisandtheirapplicationonautomates.In:SeminarsinThrombosisandHaemostasis,Vol.9,281-300(1983). - KirkwoodTBetal.
Astandardforhumanplasmin.
ThrombDiathHaemorrh34,20-30(1975). - 28thReport,WHOExpertCommitteeonBiologicalStandardization.WHOTechRepSerNo610;1977,pp13.
- FribergerPetal.
Anewspecificsubstrateforthedeterminationofplasmin.In:Chromogenicpeptidesubstrates,Eds,ScullyMFandKakkarVV,ChurchillLivingstone,121-127(1979). - GaffneyPJandMussettMV.
Internationalcollaborativestudyfortheestablishmentofthesecondinternationalreferencepreparationofplasmin.ThrombHaemost50,645-649(1983). - GaffneyPJandCurtisAD.
Theestablishmentofastandardforplasminogen.ThrombHaemost51,376-378(1984).
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2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
刚入这行,谢谢大家
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
D,K2SO4Na2CO3BaCL2NaNO3
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因