Description:
ChromogenixS-2251™isachromogenicsubstrateforplasminandstreptokinase-activatedplasminogen.Thesubstratehasbeenusedforthedeterminationof:
- Antiplasmininplasma
- Plasminogeninplasma
Composition:
EachvialcontainschromogenicsubstrateS-2251™,25mgandmannitol60mgasabulkingagent.StABIlity:Substance:Stableuntilexpirydateifstoredat2-8°C.Avoidexposuretolight.Thesubstanceishygroscopicandshouldbestoreddry.Solution:3mmol/LinH2Oisstableforatleast6monthsat2-8°C.Contaminationbymicroorganismsmaycausehydrolysis.
Chemicalname:H-D-Valyl-L-leucyl-L-lysinep-Nitroanilinedihydrochloride
Formula:H-D-Val-Leu-Lys-pNA·2HCl
Mol.wt:551.6
PlasminogenandPlasminIUandEnzymeActivity:
Plasminogen(MW:92Kda)isthezymogenformofplasmin(83KDa).
Theactivationofplasminogenisaccomplishedbyt-PA,urokinaseorstreptokinase.
Itisnotthepurposeofthistexttodescribethemechanismsofactivationofplasminogenanditsinhibition,butitisimportanttounderlinethattheycanaffectthereactionoftheactiveenzymewithchromogenicsubstrates,caseinorfibrin.
FormoreinformationrefertoGaffney(1).
1mlofnormalplasmacontainsabout180µgor2nmolofplasminogen.Theactivityofplasminogencanbeexpressedincaseinunits(CU)e.g.forexampletoSgourisetal.(2).
Friberger(3)foundthattheamountofplasminogenin1mlofnormalplasmacorrespondsto3.8CU,asdeterminedbythechromogenicmethoddescribed(streptokinaseactivatedplasminogen).
TheChromogenixplasminogenreagentwhenactivatedwithstreptokinaseshowsanactivityof 7.3nkatchromogenicsubstrateS-2251™perCU.
In1983Friberger(4)reportedthat1µgofplasmincorrespondsto0.20nkatchromogenicsubstrateS-2251™,orto0.024CUorto0.028CTA-U.
In1975,thefirststandardforplasminwasestablished(5)andtheinternationalunitwasdefinedequivalenttoCTA-U(5-6).
Thiscontainedapurifiedhumanplasminin50%glycerol.WhentestedbyFribergeretal.(7),itshowedaboutthesameactivityastheKabipreparationintermsofµmolofsubstratehydrolysedperminuteperunitofenzyme. Sincethenthedifferentaspectsofcaseinolytic,amidolyticandfibrinolyticactivityhavebeenexploredindetailsand,thediscrepancybetweenamidolyticandfibrinolyticactivityinreferencepreparationswasunderlinedasinthecaseofthe2ndInternationalReferencePreparationofplasminin1983(8).
Thecurrent3rdInternationalStandardforplasmin,97/536wasestablishedin1998,andtheresultspresentedattheFibrinolysisSubcommitteeoftheScientificandStandardizationCommitteeoftheInternationalSocietyofThrombosisandHaemostasis(Ljubljana,Slovenia,June1998).The2ndInternationalReferencePreparationwasusedasreferencematerialandachromogenicassaywasthemethodused.ThereisnoestablishedWHOstandardforplasminogen,buttheNationalInstituteforBIOLOGicalStandardsandControl,establishedtheIstBritishReferencePreparation,78/646(9).
ItisapreparationofGlu-plasminogen,assayedbyfibrinolyticandchromogenicmethodsagainstthe2ndInternationalStandardforplasmin.Completeactivationofplasminogenwasachievedbybothurokinaseorstreptokinaseandtheactivitywascomparabletothatofthereferencepreparationofplasmin.
- GaffneyPJ.
Standardizationofplasminogenassays.Haemostasis18Suppl.1,47-60(1988). - SgourisJTetal.
Thepreparationofhumanfibrinolysin(plasmin).VoxSang5,357-376(1960). - FribergerPandKnosM.
Plasminogenactivationinnormalplasma.In:Chromogenicpeptidesubstrates,Eds,ScullyMFandKakkarVV,ChurchillLivingstone,128-140(1979). - FribergerPetal.
Syntheticpeptidesubstrateassaysandfibrinolysisandtheirapplicationonautomates.In:SeminarsinThrombosisandHaemostasis,Vol.9,281-300(1983). - KirkwoodTBetal.
Astandardforhumanplasmin.
ThrombDiathHaemorrh34,20-30(1975). - 28thReport,WHOExpertCommitteeonBiologicalStandardization.WHOTechRepSerNo610;1977,pp13.
- FribergerPetal.
Anewspecificsubstrateforthedeterminationofplasmin.In:Chromogenicpeptidesubstrates,Eds,ScullyMFandKakkarVV,ChurchillLivingstone,121-127(1979). - GaffneyPJandMussettMV.
Internationalcollaborativestudyfortheestablishmentofthesecondinternationalreferencepreparationofplasmin.ThrombHaemost50,645-649(1983). - GaffneyPJandCurtisAD.
Theestablishmentofastandardforplasminogen.ThrombHaemost51,376-378(1984).
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
