Description:

TheDiapharmaFactorXKitisaninvitrodiagnostictestkitforthequantitativedeterminationofFactorXactivityinhumancitratedplasma.FactorXactivityisusefulformonitoringpatientsonoralanticoagulanttherapy(warfarin)wherebaselineINRvaluesareprolongedandINRresultsarenotreliable,suchasinOACpatientswithlupusinhibitors.TheDiaPharmaFactorXKitisalsousefulforscreeningforfactorXdeficiencies.
TheDiapharmaFactorXchromogenicassaykitallowslabstoaccuratelypredicttherapeuticwarfarinwhenINRvaluesareunreliable,suchasinthepresenceoflupusanticoagulantsorwhendirectthrombininhibitorsarediscontinued(bridgingfromDTItowarfarin).
Advantages:
- SolutiontodealingwithunstableINRsinwarfarinpatientswithlupusinhibitors
- Eliminatestheissuesofvaryingsensitivitiesofdifferentthromboplastins
- Reliable,reADIlyavailable,andeasytouseassay
- Applicationsforautomatedcoagulationanalyzers

KitComposition:
- ChromogenicSubstratePreparation25mglyophilizedFXachromogenicsubstratewithmannitoladdedasabulkingagent.Reconstitutesubstratewith20mlsterilewater.Storage&StABIlityThereconstitutedsubstrateisstablefor6monthsat2–8°C.
- Russell’sViperVenomPreparationFXactivatingproteinfromRussell’sViperVenom.ReconstitutetheRVVwith15mlsterilewater.Storage&StabilityThereconstitutedactivatorisstablefor1monthat2–8°C.
- CaCl2Preparation20mlof0.1mol/Lcalciumchloridesolution.Beforeuse,mix1volumeofRVVwith1volumeofCaCl2.Themixtureisstablefor48hoursat2–8°C.Storage&StabilityThesolutionisstableat2–8°Cuntiltheexpirydateprintedonthelabel.
- BufferPreparation100mlbuffersolutioncontaining0.05mol/LTris,pH7.8and20mg/LPolybrene®(hexadimethrinebromide).Readyforuse.Storage&StabilityThebufferisstableat2–8°Cuntiltheexpirydateprintedonthelabel.
MeasurementPrinciple:
1
| RVV | ||
| FX | → | FXa |
| Ca+2 |
2
| Chromogenic Substrate | → | Peptide+pNA(color) |
| FXa |
Themethodisbasedonatwo-stageprinciple.Instageone,FactorXisactivatedinthepresenceofcalciumtoFactorXa(FXa)bytheactivatorRussell’sViperVenom(RVV).Instagetwo,thegeneratedFXahydrolysesthechromogenicsubstrate,thusliberatingthechromophoricgroup,pNA.Thecoloristhenreadwithaspectrophotometerat405nm.ThegeneratedFXaandthustheintensityofcolorisproportionaltoptheFXactivityinthesample.
Background:
CoagulationFactorX(StuartProwerFactor,FX)isavitaminKdependentproteinproducedbytheliver.Ithasacentralpositioninthecoagulationcascade.FactorXisactivatedbothbytheextrinsicandintrinsicsystemsbeforeexertingitseffectontheconversionofprothrombintothrombin.
Patientswhoneedanticoagulationtherapycanbetreatedwithwarfarin,whichisavitaminKantagoNIST.ThistreatmentleadstoadecreaseinallvitaminKdependentclottingfactorsandthusaprolongedclottingtimeofblood.Thetherapyrequirescarefulmonitoringsinceitisessentialtofindanoptimalbalancebetweenriskofthrombosisandriskofbleedingforeachpatient. TheDiaPharmaFactorXkitisausefultoolinthemanagementofpatientswithlupusinhibitorsreceivingwarfarintherapy.
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
请问有什么方法检测细胞内活性氧更好,各位有什么经验,及相关试剂盒推荐一下。
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志

