
RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease
Complete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA
- HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes
RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.
Applications
- RemovalofRNAfromDNApreparations.1
- RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1
UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.
DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.
QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.
References
- Johnson,M.(1996)EpicentreForum3(4),7.
- Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
- delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.
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Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA. |
ORDERINFORMATION
Providedwithdilutionbuffer,10XTNEbuffer,and0.1MDTT.ebiomall.com






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(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行,谢谢大家
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3

