RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease
Complete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA- HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes
RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.
Applications
- RemovalofRNAfromDNApreparations.1
- RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1
UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.
DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.
QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.
References
- Johnson,M.(1996)EpicentreForum3(4),7.
- Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
- delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.
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| Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA. |
ORDERINFORMATION
Providedwithdilutionbuffer,10XTNEbuffer,and0.1MDTT.ebiomall.com
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刚入这行,谢谢大家
请问有什么方法检测细胞内活性氧更好,各位有什么经验,及相关试剂盒推荐一下。
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
我想检测血管组织中的钙离子浓度,不知道哪个公司有试剂盒


