
Selectorisarapid,highthroughputassaythatexploresdetergentspacetoidentifythosebestsuitedtostABIlizethemembraneproteinofinterestinadvanceofdownstreamproteincrystallizationtrials.Theassayusesdifferentialfiltrationin94differentdetergentstogenerateinformationregardingboththestabilityandthesizeoftheprotein-detergentcomplex.Thestepsofthisassayaresimple:First,theproteinisboundtoanaffinityresin,washedandelutedinanewdetergent.Theeluateisthenpassedthroughtwofilterplateswithdifferingmolecularweightcut-offs(MWCO).Thefiltrationprofilesprovidestabilityandrelativesizinginformationthatallowstheselectionofdetergentsmostlikelytostabilizeaproteininamannerthatwouldbecommensuratewithcrystallizationandstructuredetermination.
WhyshouldIexploresuchavarietyofdetergents?IntegralmembraneproteinspresentauniquechallengetoproteinchemistsandstructuralBIOLOGists.Intheirnaturalenvironment,theseproteinsexistwithinamosaiclipidbilayerthatisadynamicandcomplexenvironment.Priortostructuralstudy,however,thesemustbeextractedfromthecellmembraneandsolubilizedinsuchawayastosatisfythehydrophobicnatureofthetransmembraneregionswhileensuringthathydrophilicdomainsareincontactwithanaqueousphase.Whileasignificantnumberofdetergentsmaybecapableofkeepingtheproteininasolubleform,thestability,homogeneityandactivityofanygivenmembraneproteincanbetremendouslyaffectedbythepropertiesofthesolubilizingcomponent.
DepositstotheProteinDataBankformembraneproteinscomprisebroadrangeofdetergentanddetergent-lipidcomplexesaspartoftheproteinpreparationsleADIngthediffraction-qualitycrystals.Thus,whilecertaindetergents(DM,DDMorOG,forexample)areknownfortheirsuccessinstabilizingintegralmembraneproteinsforstructuralstudiesandarereportedathighfrequency,significanteffortwouldberequiredtoexploredetergentspace,eventocoverdetergentspreviouslysuccessfulforproteincrystallization.
WHATDOESTHISKITCONTAIN?
1xdetergentscreeningplatecontaining150µlof94detergentsat2xworkingconcentration,oneblankandonepositionforthedetergentcurrentlystabilizingtheproteinsampletobescreened
1x0.22µmfilterplatewithreceptacleplatefordetergentexchange
1xreceptacleplateintowhichtheproteinsandnewlyexchangeddetergentswillbeeluted
1x300MWCOfilterplatewithmatchedreceptacleplate
1x100MWCOfilterplatewithmatchedreceptacleplate.ebiomall.com






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通过解答,教会我,谢谢
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
2.NaNO3FeCl3AgNO3
分别有什么现象?谢谢回答!
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么

