Nucleases are widely present in the laboratory environment and can interfere with many experiments. Single-stranded RNases are ubiquitous, hard to eliminate and can rapidly degrade important samples used in microarray studies, real-time PCR, Northern blots or cDNA cloning. IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescent signal only after nuclease degradation. Assays can be used qualitatively to test lab reagents, equipment and supplies for nuclease contamination. Assays can also be used quantitatively to study enzyme kinetics. The RNaseAlert substrate employs a FAM™ (fluorescein) reporter (Em 520 nm) and a dark quencher. The RNA sequence has been carefully optimized to react with a wide variety of ribonucleases. Intact, the substrate has little or no fl uorescence. When cleaved by an RNase, the substrate fluoresces green (490 nm or UV excitation, 520 nm emission). The DNaseAlert Substrate is a synthetic DNA oligonucleotide that has a HEX™ reporter dye (hexachlorofluorescein) on one end and a dark quencher on the other end. The DNA sequence has been carefully optimized to react with a wide variety of nucleases; it contains domains that will react with single-stranded endonucleases, certain single-stranded exonucleases, and double-stranded nucleases. Intact, the substrate has little or no fluorescence. When cleaved by a DNase, the substrate fluoresces pink (536 nm or UV excitation, 556 nm emission) and can be detected visually or using a fluorometer. RNaseAlert and DNaseAlert are available in single-use tubes that are ideal for rapid hand-held visual assays at the research bench, or as bulk substrate that can be used in either visual assays or read in a fluorometer. Buffers are optimized for detection of a wide variety of DNases or RNases. RNaseAlert Kit Components:

Kit is sufficient for 25 reactions. Quality is guaranteed for six months from the date received when stored at -20°C.

bioworld(bioworld)

• 品牌所有商: BioworldTechnology,Inc.BiogotBiotechnologyCO.,Ltd
• 品牌国别: 美国
• 代表产品:  信号通路方面的一抗   AKT   MAPK   ERK   Caspase等凋亡通路   细胞周期骨架   P53   二抗   细胞因子   内参等（40字以内）  
• 品牌简介: BioworldTechnology,Inc.公司是专做代工的全球性抗体公司，总部位于美国密尼苏达，Bioworld抗体的优势主要集中在细胞信号通路及信号转导等方面,自从2008年进入中国至今
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Bioworld品牌創立以來在國內擁有4000多種常規產品現貨，其中多種規格包裝適合中國市場。Bioworld抗體的優勢主要集中在細胞信號通路及信號轉導等方面：(1)染色質調控與乙酰化信號通路(2)MAP激酶信號通路(3)SAPK/JNK信號通路(4)細胞凋亡與Caspase信號通路(5)AKT信號通路(6)轉錄與翻譯調控(7)蛋白激酶C(PKC)信號通路(8)周期調控與DNA損傷信號通路(9)細胞骨架調控通路。

bioWORLD是美国GeneLinxInternational，Inc.于1993年成立的一个部门，其使命是为世界各地的分析实验室、教育和研究机构提供优质的分子工具、生物试剂和实验室用品。我们专注于生命科学和生物技术行业的产品，专注于蛋白质纯化和蛋白质组学研究的生物固定试剂。有大量的重组蛋白和合成肽。此外，还以极具竞争力的价格提供定制肽合成和抗体生产服务。

公司总部设在俄亥俄州都柏林，销售办事处遍布全球。无论是内部制造还是国际收购，bioWorld都能以极具竞争力的价格为生物医学和生命科学行业提供产品。此外，我们还为生物技术行业的研发提供定制实验室服务和配方。