Sputum DNA Collection, Preservation and Isolation Kit is an all-in-one method designed for:

• Simple and non-invasive sputum collection; • Preservation of DNA in sputum samples at ambient temperature; • Isolation of high quality DNA.

The Sputum DNA Collection, Preservation and Isolation Kit contains 50 individual Sputum Collection and Preservation Devices, as well as the required reagents for the subsequent isolation of sputum DNA from the preserved samples. Each of the 50 individual kits consists of three components: (1) Sputum Collection Tube; (2) Collection Funnel and; (3) Norgen's Sputum DNA Preservative contained within sealed ampoules. Sputum samples (either induced or non-induced) are collected by the patient by first rinsing the mouth thoroughly with water. Following this step the patient must then cough deeply to loosen sputum and then expectorate/spit inside the Collection Funnel which has been assembled with the Collection Tube. Following collection of the sputum sample, the Collection Funnel is removed and the contents of the Preservative ampoule are then added and mixed with the collected sputum and the Collection tube is labeled. The Sputum Collection Tube is subsequently sent to the laboratory for DNA isolation and analyses. The sputum DNA in preserved samples is stable for more than two years at ambient temperature. The kit is ideal for collecting, preserving and isolating DNA samples for numerous research based applications, as well as epidemiological and population studies.

SPUTUM DNA PRESERVATIVE Norgen's Sputum DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh sputum samples. The preservative prevents the growth of Gram-positive bacteria, Gram-negative bacteria and fungi and inactivates viruses allowing the samples to be handled and shipped in a safer manner. In addition, the samples are preserved allowing their storage and shipment at ambient temperature, eliminating the requirement for refrigeration or freezing, decreasing costs. The components of the preservative will allow the collected samples to be stored for more than 2 years without any detectable DNA degradation.

NUCLEIC ACID ISOLATION FROM PRESERVED SPUTUM Sputum DNA is isolated from the preserved samples either according to ethanol precipitation or can be performed using Sputum DNA Isolation Kit (Cat#10760131-1). The preservative is also compatible with other DNA isolation methods.

FEATURES AND BENEFITS

• Non-invasive sample collection - Higher donor compliance and lower costs due to non-invasive sample collection • Convenient, all-in-one kit - Sputum sample collection, preservation and DNA isolation all with one convenient, affordable kit • Safer handling - The provided Sputum DNA Preservative is designed for rapid cellular lysis, prevents the growth of Gram-negative and Gram-positive bacteria and fungi and also inactivates viruses allowing the samples to be handled and shipped in a safer manner. • DNA is stable for years at ambient temperature - Sputum DNA preserved with Norgen's Sputum DNA Preservative is stable at room temperature for over 2 years, thereby reducing storage costs associated with fridges and freezers. • Compatible with most DNA isolation methods - DNA can be isoated from the samples via ethanol precipitation using the provided reagents. Alternatively, DNA can be isolated using other methods, such as Sputum DNA Isolation Kit (Cat# 10760131-1) which uses spin columns. • High quality DNA is suitable for sensitive downstream assays - Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR Shipping Accessories can be purchased separately - Norgen also provides shipping accessories (envelopes and bags) which meet all standards and regulations for safe shipping of biological samples.

KIT CONTENTS

1. Individual Sputum DNA Collection and Preservation Device: a. Collection Tubeb. Collection Funnelc. Preservative ampouled. Absorbant pade. Post-collection labelf. Patient instructions 2. Sputum Liquefier 3. Proteinase K 4. Purification Additive 5. Product Insert

SHELF LIFE AND HANDLINGThis kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at 4°C (stable for 2 years) or at -20oC for long term storage. All other kit components should be kept tightly sealed and stored at room temperature (15 - 25 °C) for up to 3 years without any reduction in kit performance. The Collection Tube, Collection Funnel and the Device container of each Individual Sputum DNA Collection and Preservation Device are recyclable.

bioworld(bioworld)

• 品牌所有商: BioworldTechnology,Inc.BiogotBiotechnologyCO.,Ltd
• 品牌国别: 美国
• 代表产品:  信号通路方面的一抗   AKT   MAPK   ERK   Caspase等凋亡通路   细胞周期骨架   P53   二抗   细胞因子   内参等（40字以内）  
• 品牌简介: BioworldTechnology,Inc.公司是专做代工的全球性抗体公司，总部位于美国密尼苏达，Bioworld抗体的优势主要集中在细胞信号通路及信号转导等方面,自从2008年进入中国至今
• 
  

Bioworld品牌創立以來在國內擁有4000多種常規產品現貨，其中多種規格包裝適合中國市場。Bioworld抗體的優勢主要集中在細胞信號通路及信號轉導等方面：(1)染色質調控與乙酰化信號通路(2)MAP激酶信號通路(3)SAPK/JNK信號通路(4)細胞凋亡與Caspase信號通路(5)AKT信號通路(6)轉錄與翻譯調控(7)蛋白激酶C(PKC)信號通路(8)周期調控與DNA損傷信號通路(9)細胞骨架調控通路。

bioWORLD是美国GeneLinxInternational，Inc.于1993年成立的一个部门，其使命是为世界各地的分析实验室、教育和研究机构提供优质的分子工具、生物试剂和实验室用品。我们专注于生命科学和生物技术行业的产品，专注于蛋白质纯化和蛋白质组学研究的生物固定试剂。有大量的重组蛋白和合成肽。此外，还以极具竞争力的价格提供定制肽合成和抗体生产服务。

公司总部设在俄亥俄州都柏林，销售办事处遍布全球。无论是内部制造还是国际收购，bioWorld都能以极具竞争力的价格为生物医学和生命科学行业提供产品。此外，我们还为生物技术行业的研发提供定制实验室服务和配方。