
Product Name | Sensolyte ® Cell Cytotoxicity Assay Kit |
Size | 1 kit |
Catalog # | AS-71302 |
US$ | $272 |
The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. The kit contains: Assay mixture Assay buffer Lysis solution Stop solution A detailed protocolKit Size: 500 assays | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
References | Perrot S, et al. (2003). A new nondestructive cytometric assay based on resazurin metabolism and an organ culture model for the assessment of corneal viability. Cytometry A 55, 7-14; Byth HA, et al. (2001). Assessment of a simple, non-toxic Alamar blue cell survival assay to monitor tomato cell viability. Phytochem Anal 12, 340-6; Larson EM, et al. (1997). A new, simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability. Invest Ophthalmol Vis Sci 38, 1929-33. |
Product Citations | Choi, J. et al. (2010). In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines. Bioprocess Biosyst Eng 33, 21.Leach, RE. et al. (2008). Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor. Am. J. Obs. Gyne. 198, 471e1.Wolff, GS. et al. (2007).Epidermal Growth Factor-Like Growth Factors Prevent Apoptosis of Alcohol-Exposed Human Placental Cytotrophoblast Cells.Biol Reprod. 77, 53. Armant, DR. et al (2006). Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.Development 133, 751. Kim, TH. et al. (2010). An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor. Arch Pharmacal Res 33, 1261. |
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A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
刚入这行,谢谢大家

