CEFPool(extended)(15nmol;>70%)
ProductCode:PM-CEF-E-1
PositiveControlPoolof32peptidesselectedfromdefinedHLAclassI-restrictedT-cellepitopesforTcellassays(e.g.ELISPOT).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Control
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
CEFPool(extended)(15nmol;>70%)
SelectedReferences
"StandardizationofCryopreservedPeripheralBloodMononuclearCellsThroughaRestingProcessforClinicalImmunomonitoring?DevelopmentofanAlgorithm"
Wangetal.,CytometryA.(2015)-PMID:26848928
"DichotomousEffectsofLatentCMVInfectiononthePhenotypeandFunctionalPropertiesofCD8+T-cellsandNK-cells"
Bigleyetal.,CellularImmunology(2015)-PMID:n.a
"K562-DerivedWhole-CellVaccineEnhancesAntitumorResponsesofCAR-RedirectedVirus-SpecificCytotoxicTLymphocytesInVivo"
Caruanaetal.,ClinCancerRes.(2015) -PMID:25691731
"Self-adjuvantedmRNAVaccinationinAdvancedProstateCancerPatients:aFirst-in-ManPhaseI/IIaStudy"
Kübleretal.,JImmunotherCancer.(2015)-PMID:26082837
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"ProfilingTCellActivationUsingSingle-MoleculeFluorescenceInSituHybridizationandFlowCytometry"
Bushkinetal.,J.Immunol.(2015)-PMID:25505292
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
CEFPool(extended)(15nmol;>70%)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Selectedproteins |
| Organism | Epstein-Barrvirus(HHV-4) Humancytomegalovirus(HHV-5) InfluenzaA |
| Indication/Topic | Control |
| Numberofpeptides | 32 |
FurtherInformation:
| Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
| Specifications | DefinedHLAclassI-restrictedT-cellepitopes |
| Documentation | ProtocolandDatasheet |
| Sequence | SeeProductDatasheetunder ProductDocumentation |
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刚入这行，谢谢大家
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
D,K2SO4Na2CO3BaCL2NaNO3
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
