CEFTPool(120nmol;>90%)
ProductCode:PM-CEFT-4
PositiveControlPoolof27peptidesselectedfromdefinedHLAclassIandII-restrictedT-cellepitopesforTcellassays(e.g.ELISPOT).
Amount:1vialcontaining120nmol(appr.200µg)perpeptide
Purity:>90%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Control
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
CEFTPool(120nmol;>90%)
SelectedReferences
"StandardizationofCryopreservedPeripheralBloodMononuclearCellsThroughaRestingProcessforClinicalImmunomonitoring?DevelopmentofanAlgorithm"
Wangetal.,CytometryA.(2015)-PMID:26848928
"DichotomousEffectsofLatentCMVInfectiononthePhenotypeandFunctionalPropertiesofCD8+T-cellsandNK-cells"
Bigleyetal.,CellularImmunology(2015)-PMID:n.a
"K562-DerivedWhole-CellVaccineEnhancesAntitumorResponsesofCAR-RedirectedVirus-SpecificCytotoxicTLymphocytesInVivo"
Caruanaetal.,ClinCancerRes.(2015) -PMID:25691731
"Self-adjuvantedmRNAVaccinationinAdvancedProstateCancerPatients:aFirst-in-ManPhaseI/IIaStudy"
Kübleretal.,JImmunotherCancer.(2015)-PMID:26082837
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"ProfilingTCellActivationUsingSingle-MoleculeFluorescenceInSituHybridizationandFlowCytometry"
Bushkinetal.,J.Immunol.(2015)-PMID:25505292
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
CEFTPool(120nmol;>90%)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Selectedproteins |
| Organism | Clostridiumtetani Epstein-Barrvirus(HHV-4) Humancytomegalovirus(HHV-5) InfluenzaA |
| Indication/Topic | Control |
| Numberofpeptides | 27 |
FurtherInformation:
| Amount | 1vialcontaining120nmol(appr.200µg)perpeptide |
| Specifications | DefinedHLAclassI&II-restrictedT-cellepitopes |
| Documentation | ProtocolandDatasheet |
| Sequence | SeeProductDatasheetunder ProductDocumentation |
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A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
刚入这行，谢谢大家
