Acetylome-DeacetylaseMicroarray
ProductCode:AT-MA_01
Peptidemicroarraydisplaying5599peptidesderivedfromreportedhumanacetylationsites(plus384controlpeptides).All5599peptidescarryacetylatedlysineresidues.Readoutisperformedbydifferentialmeasurement,allowingcomparativeanalysisinabsence(control)andpresenceofdeacetylase(orother)activities.Acontrolmicroarrayisprovidedwitheachorder.
Amount:1peptidemicroarray(eachpeptideisdeposited1xpersubarray,3subarraysperslide)+1controlmicroarrayperorder+dummyslidesandspacers(neededformanualincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Bindingexperiments,Substrateidentification,Acetyltransferaseassays,Acetyltransferaseinhibitorscreens
Indication(s)/Topic(s):Cancer,Acetyltransferase,Histones,Generegulationandexpression,Epigenetics,Cellsignaling,Acetylome,Deacetylase,Methyltransferase
DeliveryTime:2-5days
Acetyltransferase&DeacetylaseMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforcharacterizationofenzymessuchasacetyltransferases,methyltransferasesanddeacetylases.PeptidesareimmobilizedonglassslidesviaaflexIBLelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptides.Multipleidenticalcopiesofeachmicroarrayareproduced.Incubationcanbeperformedmanuallyorautomated.Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofJPT"sAcetyltransferase&DeacetylaseMicroarrays
-Uniquecollectionofallidentifiedhumanacetylationsites
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,lysate,phosphatase)
-Lowbackgroundonglasssurface.Read-outviafluorescence
-SavetimeforHTSassaysetup
-Screenthousandsofpotentialpeptidesubstrateseconomically
Acetylome-DeacetylaseMicroarray
SelectedReferences
"AnAcetylomePeptideMicroarrayRevealsSpecificitiesandDeacetylationSubstratesforallHumanSirtuinIsoforms"
Rauhetal,NatureCommunications(2013)-PMID:23995836
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)
Testimonial
"WehavesuccessfullyappliedJPT"speptidemicroarraysasascreeningtoolforpotentialkinasestransferingphosphatetophosphorylationsitesidentifiedbymassspectrometry.Thetriplicatealignmentaswellasthethoroughselectionofcontrolsenabledgenerationofhighqualitydataconfirmingourinvivodatageneratedbymeansofaffinitychromatographicprocedures."
MarceloP.Coba,PhD,TheWellcomeTrustSangerInstitute,Cambridge,UK
Acetylome-DeacetylaseMicroarray
| Application | Acetyltransferaseassays Substrateidentification |
| ProteinName | Selectedproteins |
| Organism | Homosapiens(Human) |
| Indication/Topic | Acetyltransferase Deacetylase Epigenetics |
| Numberofpeptides | 5599peptides(plus384controlpeptides) |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited1xpersubarray,3subarraysperslide)+1controlmicroarrayperorder+dummyslidesandspacers(neededformanualincubation) |
| Specifications | 13merpeptidesselectedformthehumanproteome |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Sequence | SeeSequenceListunderProd uctDocumentation |
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
D,K2SO4Na2CO3BaCL2NaNO3
通过解答，教会我，谢谢
