AnnotatedPhosphoSites-Tyr-Phosphatase
ProductCode:PHOS-MA-PY
Collectionof>6000phosphorylationsitesasphosphotyrosinecontainingpeptidesdisplayedononepeptidemicroarray.
Amount:2peptidemicroarrays(eachpeptideisdeposited1xpersubarray,3subarraysperslide),onefortargetincubationandoneforcontrolincubation+dummyslidesandspacers(neededformanualincubation)
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Phosphositedetection,Substrateidentification
Indication(s)/Topic(s):Phosphatase
DeliveryTime:10-12days
PhosphataseSubstrateMicroarray
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforcharacterizationofenzymetargetfamiliessuchasphosphatases.PhosphorylatedpeptidesareimmobilizedonglassslidesviaaflexIBLelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptides.Multipleidenticalcopiesofeachmicroarrayareproduced.Incubationcanbeperformedmanuallyorautomated.Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofJPT"sPhosphataseSubstrateMicroarray
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,lysate,phosphatase)
-Lowbackgroundonglasssurface.Read-outviafluorescence
-SavetimeforHTSassaysetup
-Screenthousandsofpotentialpeptidesubstrateseconomically
AnnotatedPhosphoSites-Tyr-Phosphatase
SelectedReferences
"PhosphopeptideDetectionwithBiotin-LabeledPhos-tag"
Kinoshita-Kikutaetal.,Methodsinmolecularbiology (2016)-PMID:26584916
"PhosphotyrosineSubstrateSequenceMotifsforDualSpecificityPhosphatases"
Zhaoetal.,PLoSOne.(2015)-PMID:26302245
"Phos-tagTechnologyforKinomics"
Kinoshita-Kikutaetal.,Kinomics:ApproachesandApplications(2015)-PMID:n.a.
"CharacterizationoftheHostResponsetoPichindeVirusInfectionintheSyrianGoldenHamsterbySpecies-SpecificKinomeAnalysis"
Falcinellietal.,Mol.Cell.Proteomics.(2015)-PMID:25573744
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)
Testimonial
"WehavesuccessfullyappliedJPT"speptidemicroarraysasascreeningtoolforpotentialkinasestransferingphosphatetophosphorylationsitesidentifiedbymassspectrometry.Thetriplicatealignmentaswellasthethoroughselectionofcontrolsenabledgenerationofhighqualitydataconfirmingourinvivodatageneratedbymeansofaffinitychromatographicprocedures."
MarceloP.Coba,PhD,TheWellcomeTrustSangerInstitute,Cambridge,UK
AnnotatedPhosphoSites-Tyr-Phosphatase
| Application | Phosphositedetection Substrateidentification |
| ProteinName | Selectedproteins |
| Organism | Homosapiens(Human) |
| Indication/Topic | Phosphatase |
| Numberofpeptides | >6000 |
FurtherInformation:
| Amount | 2peptidemicroarrays(eachpeptideisdeposited1xpersubarray,3subarraysperslide),onefortargetincubationandoneforcontrolincubation+dummyslidesandspacers(neededformanualincubation) |
| Specifications | Humanphosphopeptides(13meric)containingonephosphorylatedtyrosineresidueinthecenterposition |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Sequence | SeeDatasheetunderProduct Documentation |
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A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
刚入这行，谢谢大家
