PhosphorylationSite-DetectorNKTK
ProductCode:PD-072
Peptidemicroarraydisplaying208peptidesderivedfromapeptidescan(15merswith8aaoverlap)throughNKTK(Swiss-ProtID:P30414)ofHomosapiens(Human).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Phosphositedetection,Substrateidentification
Indication(s)/Topic(s):Kinase
DeliveryTime:10-12days
PhosphorylationSite-Detectors
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesenablingcharacterizationofenzymessuchaskinasesaswellasmethyl,glycosyl,acetyltransferases.PeptidesareimmobilizedonglassslidesviaaflexIBLelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptidesinfouridenticalsubarrays(3copiesofpeptidewithineachsubarray).Incubationcanbeperformedmanuallyorautomated.
BenefitsofJPT"sPhosphorylationSite-Detector
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourenzymepreparationisneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,lysate,kinase)
-Lowbackgroundonglasssurface
-Read-outusingfluorescenctlylabeledantibodiesorrADIoisotopicallylabeledATP
-Screenthousandsofpotentialpeptidesubstrateseconomically
PhosphorylationSite-DetectorNKTK
SelectedReferences
"Parvulin17-CatalyzedTubulinPolymerizationisRegulatedbyCalmodulininaCalcium-DependentManner"
Burgardtetal,JBiolChem.(2015)-PMID:25940090
"PhosphopeptideDetectionwithBiotin-LabeledPhos-tag"
Kinoshita-Kikutaetal.,MethodsinMolecularBiology (2016)-PMID:26584916
"PKc-thetaisaNovelsc35SplicingFactorRegulatorinResponsetoTcellActivation"
McCuaigetal.,FrontiersinImmunology -PMID: n.a.
"Phos-tagTechnologyforKinomics"
Kinoshita-Kikutaetal.,Kinomics:ApproachesandApplications(2015)-PMID:n.a.
"CharacterizationoftheHostResponsetoPichindeVirusInfectionintheSyrianGoldenHamsterbySpecies-SpecificKinomeAnalysis"
Falcinellietal.,Mol.Cell.Proteomics.(2015)-PMID:25573744
"AntiviralPotentialofERK/MAPKandPI3K/AKT/mTORSignalingModulationforMiddleEastRespiratorySyndromeCoronavirusInfectionasIdentifiedbyTemporalKinomeAnalysis"
Kindrachuketal.,AntimicrobAgentsChemother.(2015)-PMID:25487801
ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)
Testimonial
"WithJPT´speptidemicroarrayplatform,high-throughputkinaseprofilingusingsmallamountsofcelllysatebecomesfeasible,providingaccesstothediscoveryandmonitoringofnovelbioMarkersinParkinson"sDisease."
JeremyNichols,Parkinsons"sInstitute,Sunnyvale,USA
PhosphorylationSite-DetectorNKTK
| Application | Phosphositedetection Substrateidentification |
| ProteinName | NKTR/NK-tumorrecognitionprotein |
| Organism | Homosapiens(Human) |
| Indication/Topic | Kinase |
| Numberofpeptides | 208 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededformanualincubation) |
| Specifications | Peptidescan(15merswith8aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P30414 |
| Sequence | MGAQDRPQCHFDIEINREPVGRIMFQLF SDICPKTCKNFLCLCSGEKGLGKTTGKK LCYKGSTFHRVVKNFMIQGGDFSEGNGK GGESIYGGYFKDENFILKHDRAFLLSMA NRGKHTNGSQFFITTKPAPHLDGVHVVF GLVISGFEVIEQIENLKTDAASRPYADV RVIDCGVLATKSIKDVFEKKRKKPTHSE GSDSSSNSSSSSESSSESELEHERSRRR KHKRRPKVKRSKKRRKEASSSEEPRNKH AMNPKGHSERSDTNEKRSVDSSAKREKP VVRPEEIPPVPENRFLLRRDMPVVTAEP EPKIPDVAPIVSDQKPSVSKSGRKIKGR GTIRYHTPPRSRSCSESDDDDSSETPPH WKEEMQRLRAYRPPSGEKWSKGDKLSDP CSSRWDERSLSQRSRSWSYNGYYSDLST ARHSGHHKKRRKEKKVKHKKKGKKQKHC RRHKQTKKRRILIPSDIESSKSSTRRMK SSCDRERSSRSSSLSSHHSSKRDWSKSD KDVQSSLTHSSRDSYRSKSHSQSYSRGS SRSRTASKSSSHSRSRSKSRSSSKSGHR KRASKSPRKTASQLSENKPVKTEPLRAT MAQNENVVVQPVVAENIPVIPLSDSPPP SRWKPGQKPWKPSYERIQEMKAKTTHLL PIQSTYSLANIKETGSSSSYHKREKNSE SDQSTYSKYSDRSSESSPRSRSRSSRSR SYSRSYTRSRSLASSHSRSRSPSSRSHS RNKYSDHSQCSRSSSYTSISSDDGRRAK RRLRSSGKKNSVSHKKHSSSSEKTLHSK YVKGRDRSSCVRKYSESRSSLDYSSDSE QSSVQATQSAQEKEKQGQMERTHNKQEK NRGEEKSKSERECPHSKKRTLKENLSDH LRNGSKPKRKNYAGSKWDSESNSERDVT KNSKNDSHPSSDKEEGEATSDSESEVSE IHIKVKPTTKSSTNTSLPDDNGAWKSSK QRTSTSDSEGSCSNSENNRGKPQKHKHG SKENLKREHTKKVKEKLKGKKDKKHKAP KRKQAFHWQPPLEFGEEEEEEIDDKQVT QESKEKKVSENNETIKDNILKTEKSSEE DLSGKHDTVTVSSDLDQFTKDDSKLSIS PTALNTEENVACLQNIQHVEESVPNGVE DVLQTDDNMEICTPDRSSPAKVEETSPL GNARLDTPDINIVLKQDMATEHPQAEVV KQESSMSESKVLGEVGKQDSSSASLASA GESTGKKEVAEKSQINLIDKKWKPLQGV GNLAAPNAATSSAVEVKVLTTVPEMKPQ GLRIEIKSKNKVRPGSLFDEVRKTARLN RRPRNQESSSDEQTPSRDDDSQSRSPSR SRSKSETKSRHRTRSVSYSHSRSRSRSS TSSYRSRSYSRSRSRGWYSRGRTRSRSS SYRSYKSHRTSSRSRSRSSSYDPHSRSR SYTYDSYYSRSRSRSRSQRSDSYHRGRS YNRRSRSCRSYGSDSESDRSYSHHRSPS ESSRYS |
| ProteinLength(aa) | 1462 |
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A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
通过解答，教会我，谢谢
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
