PhosphorylationSite-DetectorRAF1
ProductCode:PD-056
Peptidemicroarraydisplaying319peptidesderivedfromapeptidescan(13merswith11aaoverlap)throughRAF1(Swiss-ProtID:P04049)ofHomosapiens(Human).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Phosphositedetection,Substrateidentification
Indication(s)/Topic(s):Kinase
DeliveryTime:10-12days
PhosphorylationSite-Detectors
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesenablingcharacterizationofenzymessuchaskinasesaswellasmethyl,glycosyl,acetyltransferases.PeptidesareimmobilizedonglassslidesviaaflexIBLelinker.Chemoselectivecouplinggeneratesmicroarraysdisplayingcovalentlyattachedpeptidesinfouridenticalsubarrays(3copiesofpeptidewithineachsubarray).Incubationcanbeperformedmanuallyorautomated.
BenefitsofJPT"sPhosphorylationSite-Detector
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourenzymepreparationisneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,lysate,kinase)
-Lowbackgroundonglasssurface
-Read-outusingfluorescenctlylabeledantibodiesorrADIoisotopicallylabeledATP
-Screenthousandsofpotentialpeptidesubstrateseconomically
PhosphorylationSite-DetectorRAF1
SelectedReferences
"Parvulin17-CatalyzedTubulinPolymerizationisRegulatedbyCalmodulininaCalcium-DependentManner"
Burgardtetal,JBiolChem.(2015)-PMID:25940090
"PhosphopeptideDetectionwithBiotin-LabeledPhos-tag"
Kinoshita-Kikutaetal.,MethodsinMolecularBiology (2016)-PMID:26584916
"PKc-thetaisaNovelsc35SplicingFactorRegulatorinResponsetoTcellActivation"
McCuaigetal.,FrontiersinImmunology -PMID: n.a.
"Phos-tagTechnologyforKinomics"
Kinoshita-Kikutaetal.,Kinomics:ApproachesandApplications(2015)-PMID:n.a.
"CharacterizationoftheHostResponsetoPichindeVirusInfectionintheSyrianGoldenHamsterbySpecies-SpecificKinomeAnalysis"
Falcinellietal.,Mol.Cell.Proteomics.(2015)-PMID:25573744
"AntiviralPotentialofERK/MAPKandPI3K/AKT/mTORSignalingModulationforMiddleEastRespiratorySyndromeCoronavirusInfectionasIdentifiedbyTemporalKinomeAnalysis"
Kindrachuketal.,AntimicrobAgentsChemother.(2015)-PMID:25487801
ApplicationNotesforEnzymeSubstrateMicroarrays
"Exploringthehumanacetylomeusinghigh-densitypeptidemicroarrays"
Maschetal.,ApplicationNote(2012)(fulltext)
"UnravelingPhosphorylationoftheSplicingFactorSC35bytheDualSignalingKinasePKCinHumanT-Cells"
Raoetal.,ApplicationNote(2016)(fulltext)
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistoneModifyingEnzymeComplex"
Wilczeketal.,ApplicationNote(2013)(fulltext)
Testimonial
"WithJPT´speptidemicroarrayplatform,high-throughputkinaseprofilingusingsmallamountsofcelllysatebecomesfeasible,providingaccesstothediscoveryandmonitoringofnovelbioMarkersinParkinson"sDisease."
JeremyNichols,Parkinsons"sInstitute,Sunnyvale,USA
PhosphorylationSite-DetectorRAF1
| Application | Phosphositedetection Substrateidentification |
| ProteinName | RAF1 |
| Organism | Homosapiens(Human) |
| Indication/Topic | Kinase |
| Numberofpeptides | 319 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededformanualincubation) |
| Specifications | Peptidescan(13merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P04049 |
| NCBI | NP_004575 |
| Sequence | MEHIQGAWKTISNGFGFKDAVFDGSSCI SPTIVQQFGYQRRASDDGKLTDPSKTSN TIRVFLPNKQRTVVNVRNGMSLHDCLMK ALKVRGLQPECCAVFRLLHEHKGKKARL DWNTDAASLIGEELQVDFLDHVPLTTHN FARKTFLKLAFCDICQKFLLNGFRCQTC GYKFHEHCSTKVPTMCVDWSNIRQLLLF PNSTIGDSGVPALPSLTMRRMRESVSRM PVSSQHRYSTPHAFTFNTSSPSSEGSLS QRQRSTSTPNVHMVSTTLPVDSRMIEDA IRSHSESASPSALSSSPNNLSPTGWSQP KTPVPAQRERAPVSGTQEKNKIRPRGQR DSSYYWEIEASEVMLSTRIGSGSFGTVY KGKWHGDVAVKILKVVDPTPEQFQAFRN EVAVLRKTRHVNILLFMGYMTKDNLAIV TQWCEGSSLYKHLHVQETKFQMFQLIDI ARQTAQGMDYLHAKNIIHRDMKSNNIFL HEGLTVKIGDFGLATVKSRWSGSQQVEQ PTGSVLWMAPEVIRMQDNNPFSFQSDVY SYGIVLYELMTGELPYSHINNRDQIIFM VGRGYASPDLSKLYKNCPKAMKRLVADC VKKVKEERPLFPQILSSIELLQHSLPKI NRSASEPSLHRAAHTEDINACTLTTSPR LPVF |
| ProteinLength(aa) | 648 |
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
D,K2SO4Na2CO3BaCL2NaNO3
通过解答，教会我，谢谢
