MultiwellRepliTope™EBV(EBNA3c)
ProductCode:RT-MW-EBV-EBNA3c
Peptidemicroarraydisplaying265peptidesin3x7subarrays(3copies/subarray)forincubationwith6samples(andonecontrol)inparallelusinganArraySlide24-4chamber.Apeptidescan(15merswith11aaoverlap)throughEpstein-Barrnuclearantigen6(EBNA3c)(Swiss-ProtID:Q69140)ofEpstein-Barrvirus(HHV4)isdisplayed.
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,3x7subarraysperslide)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SEROMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,Cancer,Hodgkin"slymphoma,Burkitt"slymphoma,Nasopharyngealcarcinoma,Dermatomyositis,Systemiclupuserythematosus,Rheumatoidarthritis,Sjgren"ssyndrome,Multiplesclerosis
DeliveryTime:10-12days
FortheincubationofMultiwellRepliTope™MicroarrayswithdifferentsamplesanincubationchambersuchasArrayslidechamber24-4isneeded!
JPT"sRepliTope™MultiwellPeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunemonitoring,detectionandvalidationofproteininteractions.OverlappingpeptidesareimmobilizedonglassslidesviaaflexIBLelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinthreecopiespersubarrayoneachslide.The3x7subarrayscanbeincubatedwithdifferentsamplesusinganArrayslidechamber24-4andregularELISAequipment(washer,incubator).Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofMultiwellRepliTope™PeptideMicroarrays
-Incubateseveraldifferentsamplesononemicroarrayslide
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence
MultiwellRepliTope™EBV(EBNA3c)
SelectedReferencesforJPT"sPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684
ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext
TestimonialsforJPT"sPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.OurABIlitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPT"scommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada
"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPT"sPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!"
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA
"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPT"sPepStar™peptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.HoffmannLaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland
MultiwellRepliTope™EBV(EBNA3c)
| Application | Antibodyepitopediscovery Immunemonitoring |
| ProteinName | Epstein-Barrnuclearantigen(EBNA) |
| Organism | Epstein-Barrvirus(HHV-4) |
| Indication/Topic | Autoimmunedisease Burkitt"slymphoma Hodgkin"slymphoma |
| Numberofpeptides | 265 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide) |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | Q69140 |
| NCBI | YP_001129465 |
| Sequence | MESFEGEGDSIQSPDNARGDDVQNTGEH IQDPGPGPSTGGASEGLVQNEPDSRDQQ SRGQRRGDENRGWMQRIRRRRRRRAALS GHLLDMEDNVPPWFPPHDITPYVARNIR DAACQAVKHSHLQALSNLILDSGLDTQH LLCFVMAARQRLQDIRRGPLVVEGGVGW RHWLLTSPSRSWSMGYRTATLRTLTPVP NRVGADSIMLTATFGCQNGALAINTFSA TVWIPPPAGPREQERYAREAEVRFLRGK WQRRFRRIFDLIELCGSLHHVWQNMLQT EENLLDFVRFMGVMSSCNSSSVNYWFHK TIGNFKPYYPWNAPPNENPYHARRGIKE QVIQKAFLKAQRQGLSMLATGGGPRGDA TSETSSDEDTGRQGSDVELESSDDELPY IDPNMEPVQQRPVMFVSRVPVRKPRTLP WPTPKTHPVKRTIVKTSYRSDEAEEAQS TPERPGPSKQPSEPVEPAHTTPAGRSTV ILHEPPREPEAVSFKPPPPPSRRRRGAC VVYDDDIIEVIDVETTEEETTSMQRQPP LGQQPPPPVISTGSAMSSSHTDPSVTQP SKPHRKPQDGFQRSGRRQKRAMPPPVSP SDAGPPSTRPRVMAPPSTGPRVMATPST GPRDMAPPSTGPRDMAPPSTGPRDMAPP STGPRDMAPTVVHMFTRERLLTQSTGPA PRSFWEMRAGRDAPKIQQEPSSQQQPAT QSTPPCQSWVPSVYVLPAVDAGNAQPLQ ISHLSSMSPTQPISHEEQPRYEDPDTPL DLSLHPDTATLPPTQDLYPGREDLQATQ APYPGYEEPRPPQAPFVGDYGFVQIPSA QWEPHPSQGTYQGHIDPQLPAALDLGPE QPRFPQDPYVYSGGQLSSCPGYAGPWPS RPQHPRYRHTLALWPREPRHGHSQGPWK PWSAHLPPQWDGSAGHGQDQVSQFPHLH SETGPPRLQLSSVPQVLYPQPLVSSSAP SWSSPQPRAPIRPIPTRFPPPPMPLQDS MAVGCDSSGTACPSMPFASDYSQGAFTP LDINAPTPKSPRVEESSHGPARCSQATS EAQEILSDNSEISVFPKDAKQTDYDAST ESELD |
| ProteinLength(aa) | 1069 |
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（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
