MultiwellRepliTope™EBV(GP350/GP340)
ProductCode:RT-MW-EBV-GP350/GP340
Peptidemicroarraydisplaying224peptidesin3x7subarrays(3copies/subarray)forincubationwith6samples(andonecontrol)inparallelusinganArraySlide24-4chamber.Apeptidescan(15merswith11aaoverlap)throughEnvelopeglycoproteinGP350/GP340(Swiss-ProtID:P03200)ofEpstein-Barrvirus(HHV4)isdisplayed.
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,3x7subarraysperslide)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SEROMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,Cancer,Hodgkin"slymphoma,Burkitt"slymphoma,Nasopharyngealcarcinoma,Dermatomyositis,Systemiclupuserythematosus,Rheumatoidarthritis,Sjgren"ssyndrome,Multiplesclerosis
DeliveryTime:10-12days
FortheincubationofMultiwellRepliTope™MicroarrayswithdifferentsamplesanincubationchambersuchasArrayslidechamber24-4isneeded!
JPT"sRepliTope™MultiwellPeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunemonitoring,detectionandvalidationofproteininteractions.OverlappingpeptidesareimmobilizedonglassslidesviaaflexIBLelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinthreecopiespersubarrayoneachslide.The3x7subarrayscanbeincubatedwithdifferentsamplesusinganArrayslidechamber24-4andregularELISAequipment(washer,incubator).Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofMultiwellRepliTope™PeptideMicroarrays
-Incubateseveraldifferentsamplesononemicroarrayslide
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence
MultiwellRepliTope™EBV(GP350/GP340)
SelectedReferencesforJPT"sPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684
ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext
TestimonialsforJPT"sPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.OurABIlitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPT"scommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada
"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPT"sPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!"
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA
"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPT"sPepStar™peptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.HoffmannLaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland
MultiwellRepliTope™EBV(GP350/GP340)
| Application | Antibodyepitopediscovery Immunemonitoring |
| ProteinName | Envelopeglycoprotein |
| Organism | Epstein-Barrvirus(HHV-4) |
| Indication/Topic | Autoimmunedisease Burkitt"slymphoma Hodgkin"slymphoma |
| Numberofpeptides | 224 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide) |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P03200 |
| Sequence | MEAALLVCQYTIQSLIHLTGEDPGFFNV EIPEFPFYPTCNVCTADVNVTINFDVGG KKHQLDLDFGQLTPHTKAVYQPRGAFGG SENATNLFLLELLGAGELALTMRSKKLP INVTTGEEQQVSLESVDVYFQDVFGTMW CHHAEMQNPVYLIPETVPYIKWDNCNST NITAVVRAQGLDVTLPLSLPTSAQDSNF SVKTEMLGNEIDIECIMEDGEISQVLPG DNKFNITCSGYESHVPSGGILTSTSPVA TPIPGTGYAYSLRLTPRPVSRFLGNNSI LYVFYSGNGPKASGGDYCIQSNIVFSDE IPASQDMPTNTTDITYVGDNATYSVPMV TSEDANSPNVTVTAFWAWPNNTETDFKC KWTLTSGTPSGCENISGAFASNRTFDIT VSGLGTAPKTLIITRTATNATTTTHKVI FSKAPESTTTSPTLNTTGFADPNTTTGL PSSTHVPTNLTAPASTGPTVSTADVTSP TPAGTTSGASPVTPSPSPWDNGTESKAP DMTSSTSPVTTPTPNATSPTPAVTTPTP NATSPTPAVTTPTPNATSPTLGKTSPTS AVTTPTPNATSPTLGKTSPTSAVTTPTP NATSPTLGKTSPTSAVTTPTPNATGPTV GETSPQANATNHTLGGTSPTPVVTSQPK NATSAVTTGQHNITSSSTSSMSLRPSSN PETLSPSTSDNSTSHMPLLTSAHPTGGE NITQVTPASISTHHVSTSSPAPRPGTTS QASGPGNSSTSTKPGEVNVTKGTPPQNA TSPQAPSGQKTAVPTVTSTGGKANSTTG GKHTTGHGARTSTEPTTDYGGDSTTPRP RYNATTYLPPSTSSKLRPRWTFTSPPVT TAQATVPVPPTSQPRFSNLSMLVLQWAS LAVLTLLLLLVMADCAFRRNLSTSHTYT TPPYDDAETYV |
| ProteinLength(aa) | 907 |
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刚入这行，谢谢大家
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
