MultiwellRepliTope™HAdV-5(pentonprotein)
ProductCode:RT-MW-HAdV5
Peptidemicroarraydisplaying140peptidesin21subarrays(3copies/subarray)forincubationwith21samplesinparallelusinganArraySlide24-4chamber.Apeptidescan(15merswith11aaoverlap)throughPentonprotein(Swiss-ProtID:P12538)ofHumanadenovirus5(HAdV-5)isdisplayed.
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SEROMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,Respiratoryinfection
DeliveryTime:10-12days
FortheincubationofMultiwellRepliTope™MicroarrayswithdifferentsamplesanincubationchambersuchasArrayslidechamber24-4isneeded!
JPT"sRepliTope™MultiwellPeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunemonitoring,detectionandvalidationofproteininteractions.OverlappingpeptidesareimmobilizedonglassslidesviaaflexIBLelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinthreecopiespersubarrayoneachslide.The21subarrayscanbeincubatedwithdifferentsamplesusinganArrayslidechamber24-4andregularELISAequipment(washer,incubator).Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofMultiwellRepliTope™PeptideMicroarrays
-Incubateseveraldifferentsamplesononemicroarrayslide
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence
MultiwellRepliTope™HAdV-5(pentonprotein)
SelectedReferencesforJPT"sPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684
ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext
TestimonialsforJPT"sPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.OurABIlitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPT"scommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada
"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPT"sPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!"
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA
"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPT"sPepStar™peptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.HoffmannLaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland
MultiwellRepliTope™HAdV-5(pentonprotein)
| Application | Antibodyepitopediscovery Immunemonitoring |
| ProteinName | Pentonprotein |
| Organism | Humanadenovirus |
| Indication/Topic | Respiratoryinfection |
| Numberofpeptides | 140 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide) |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P12538 |
| NCBI | AP_000206 |
| Sequence | MRRAAMYEEGPPPSYESVVSAAPVAAAL GSPFDAPLDPPFVPPRYLRPTGGRNSIR YSELAPLFDTTRVYLVDNKSTDVASLNY QNDHSNFLTTVIQNNDYSPGEASTQTIN LDDRSHWGGDLKTILHTNMPNVNEFMFT NKFKARVMVSRLPTKDNQVELKYEWVEF TLPEGNYSETMTIDLMNNAIVEHYLKVG RQNGVLESDIGVKFDTRNFRLGFDPVTG LVMPGVYTNEAFHPDIILLPGCGVDFTH SRLSNLLGIRKRQPFQEGFRITYDDLEG GNIPALLDVDAYQASLKDDTEQGGGGAG GSNSSGSGAEENSNAAAAAMQPVEDMND HAIRGDTFATRAEEKRAEAEAAAEAAAP AAQPEVEKPQKKPVIKPLTEDSKKRSYN LISNDSTFTQYRSWYLAYNYGDPQTGIR SWTLLCTPDVTCGSEQVYWSLPDMMQDP VTFRSTRQISNFPVVGAELLPVHSKSFY NDQAVYSQLIRQFTSLTHVFNRFPENQI LARPPAPTITTVSENVPALTDHGTLPLR NSIGGVQRVTITDARRRTCPYVYKALGI VSPRVLSSRTF |
| ProteinLength(aa) | 571 |
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刚入这行，谢谢大家
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
