HistonePeptideH2B106:125K108ac(H2B.11A1B1D1H1L1M1N1O2E2F3B)
ProductCode:SP-His_0046
C-terminallybiotinylatedsyntheticHistonepeptidecarrying1modification(K108ac)derivedfromhumanHistoneH2B,H2B.11A1B1D1H1L1M1N1O2E2F3B(Swiss-ProtId(s):P62807,Q99880,Q99879,Q93079,Q99877,P23527,Q16778,P33778,Q5QNW6,Q8N257,Q96A08,P58876).
Aminoacidsequence:Ac-LA-Lys(Ac)-HAVSEGTKAVTKYTSSK-Lys(Biotinoyl)-NH2
Amount:1vialcontaining10nmol(appr.35µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):WesternBlot,Bindingexperiments,ELISA,Enzymekinetics,Pulldownassays,SubstrateSpecifity
Indication(s)/Topic(s):Cancer,Phosphatase,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulation&expression,Epigenetics,Cellsignaling,Nucleosome,Aging
Deliverytime:2-5days
JPT"sHistoneCodePeptides
Ashistonesareamajorfocusofepigenetics,JPT"scomputationalscientistscompiledauniquelibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualbiotinylatedpeptides.Thepeptidelibraryconsistsofmorethansevenhundred20merpeptidesfrom29histonefamilies(e.g.H2A[1,19,85...],H2B[1,22,42...],H3[1,23,43...],H4[1,14,44...]).PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation,citrullination)invariouscombinationsrepresentingnaturalvariants.JPT"shighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPT"scatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.
BenefitsofJPT"sHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningthewholeHistoneCodePeptidelibraryisalsoavailabledisplayedonpeptidemicroarrays
-ProvisionoffreezedriedaliquotsforenhancedstABIlity
-Proventrackrecordformanyapplications
HistonePeptideH2B106:125K108ac(H2B.11A1B1D1H1L1M1N1O2E2F3B)
SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhaBDitiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)
ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)
TestimonialforHistoneCodePeptideMicroarray
"JPT"snewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)
HistonePeptideH2B106:125K108ac(H2B.11A1B1D1H1L1M1N1O2E2F3B)
| HistoneFamily | HistoneH2B |
| Modification | Acetylation |
| SequencePosition | Core |
| Isoform | H2B.1 H2B.1A H2B.1B H2B.1D H2B.1H H2B.1L H2B.1M H2B.1N H2B.1O H2B.2E H2B.2F H2B.3B |
| Biotinylation | BiotinylatedPeptide |
| Application | ELISA |
| ProteinName | Histones |
| Organism | Homosapiens(Human) |
| Indication/Topic | Acetyltransferase Epigenetics Kinase |
| Numberofpeptides | 1 |
FurtherInformation:
| Amount | 1vialcontaining10nmol(appr.35µg) |
| Specifications | C-terminallybiotinylatedsyntheticHistonepeptidecarrying1modification(K108ac) |
| Documentation | ProductDatasheet |
| Swiss-ProtID | P62807, Q99880, Q99879, Q93079, Q99877, P23527, Q16778, P33778, Q5QNW6, Q8N257, Q96A08, P58876 |
| Sequence | Ac-LA-Lys(Ac)-HAVSEGTKAVTKYT SSK-Lys(Biotinoyl)-NH2 |
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刚入这行，谢谢大家
请问有什么方法检测细胞内活性氧更好，各位有什么经验，及相关试剂盒推荐一下。
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
